Evidence map›Paper›PMID 42039666›Full record

ArticlebioRxiv : the preprint server for biology2026

Spectroscopic DNA-PAINT for simultaneous multiplexed super-resolution microscopy.

Md Abul Shahid, Kripa Patel, David A Miller, Yang Zhang

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

4 authors.

Md Abul ShahidMolecular Analytics and Photonics (MAP) Lab, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606.
Kripa PatelMolecular Analytics and Photonics (MAP) Lab, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606.
David A MillerDepartment of Biomedical Engineering, Duke University, Durham, NC 27708.
Yang ZhangMolecular Analytics and Photonics (MAP) Lab, Department of Textile Engineering, Chemistry and Science, North Carolina State University, Raleigh, NC 27606.ORCID 0000-0003-1011-3001

Funding

Developing Switchable and Functional Fluorophores For Multi-Functional Super-Resolution MicroscopyR35GM155241 · NIGMS · NORTH CAROLINA STATE UNIVERSITY RALEIGH · PI Yang Zhang · 2024 to 2026
$1.1M
Bright and switchable fluorophores for highly multiplexed super-resolution microscopy towards molecular interaction imagingR21GM141675 · NIGMS · NORTHWESTERN UNIVERSITY · PI ZHANG, YANG · 2021 to 2022
$400k
NIGMS NIH HHS R21 GM141675NIGMS NIH HHS R35 GM155241
6 · The paper itself

Abstract

Simultaneous multiplexed super-resolution imaging remains a central challenge in single-molecule localization microscopy (SMLM), due to the limited number of spectrally distinguishable fluorophores and the trade-off between spatial and spectral precision. Here, we introduce spectroscopic DNA-PAINT (sDNA-PAINT), a framework that integrates DNA-PAINT with spectroscopic SMLM (sSMLM) to enable simultaneous multiplexed imaging with high spatial and spectral fidelity. Using DNA Origami Nanorulers,

Indexed as

DNA-PAINTmultiplexingsingle-molecule spectroscopysuper-resolution microscopy

Identifiers

PMID42039666
PMCPMC13104853

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.