Evidence map›Paper›PMID 42039511›Full record

ArticlebioRxiv : the preprint server for biology2026

NaP-TRAP: A versatile and accessible workflow to dissect principles of translational regulation and mRNA stability.

Amit Gupta, Anna Z Struba, Srihari Madhavan, Ethan Strayer, Jean-Denis Beaudoin

Abstract readPreprint
PubMed Full text at PMC Publisher
In one paragraph

Article in bioRxiv : the preprint server for biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

5 authors.

Amit GuptaDepartment of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA.
Anna Z StrubaDepartment of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA.ORCID 0000-0001-6178-0205
Srihari MadhavanDepartment of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA.
Ethan StrayerDepartment of Genetics, Yale University School of Medicine, New Haven, CT, USA.ORCID 0000-0003-3612-741X
Jean-Denis BeaudoinDepartment of Genetics and Genome Sciences, UConn Health, Farmington, CT, USA.ORCID 0000-0003-4932-1668

Funding

Regulatory roles of the epitranscriptome and RNA structurome during vertebrate developmentR35GM146883 · NIGMS · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Jean-Denis Beaudoin · 2022 to 2026
$2.1M
The UConn/JAX-GM Training Program in Genomic ScienceT32HG010463 · NHGRI · UNIVERSITY OF CONNECTICUT SCH OF MED/DNT · PI Mark D ADAMS, Christine R Beck · 2020 to 2026
$1.5M
NHGRI NIH HHS T32 HG010463NIGMS NIH HHS R35 GM146883
6 · The paper itself

Abstract

The translation of mRNA into protein is tightly regulated by both cellular Basic Protocol 1: Design, assembly, and synthesis of NaP-TRAP reporter libraries. Support Protocol 1: Design, assembly, and synthesis of NaP-TRAP individual reporters and spike-ins. Basic Protocol 2: NaP-TRAP delivery by micro-injection in zebrafish embryos. Alternate Protocol 1: NaP-TRAP delivery by transfection in cultured mammalian cells. Basic Protocol 3: NaP-TRAP pulldown and RNA extraction. Basic Protocol 4: Preparation of NaP-TRAP cDNA Sequencing Libraries. Alternate Protocol 2: NaP-TRAP-qPCR module for low-cost validation. Basic Protocol 5: Computational analysis of NaP-TRAP MPRA data.

Indexed as

Gene expressionMassively Parallel Reporter AssayPost-transcriptional regulationTranslation

Identifiers

PMID42039511
PMCPMC13105144

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.