Evidence map›Paper›PMID 42038129›Full record

ArticleGalen medical journal2024

Evaluation of Sperm DNA Fragmentation in Oligoasthenoteratozoospermia Patients Using Two Different Techniques: TUNEL and Sperm Chromatin Dispersion Assays.

Raziye Chegini, Mahshad Khodarahmian, Niloufar Ahmadian, Sadegh Shirian, Farnaz Khadivi, Shahrzad Zhaeentan, Maryam Salem, Narjes Feizollahi, Azim Hedayatpour, Mehdi Abbasi

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Article in Galen medical journal, 2024. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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0cells of the map it votes in
1citing papers in PubMed
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1 · What the graph read from it

What it found

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

10 authors.

Raziye CheginiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mahshad KhodarahmianDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Niloufar AhmadianDepartment of Urology, faculty of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Sadegh ShirianDepartment of Pathobiology, School of Veterinary Medicine, Shahrekord University, Shahrekord, Iran.
Farnaz KhadiviShiraz Molecular Pathology Research Center, Dr Daneshbod Path Lab, Shiraz, Iran.
Shahrzad ZhaeentanDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Maryam SalemDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Narjes FeizollahiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Azim HedayatpourDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.
Mehdi AbbasiDepartment of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroundOligoasthenoteratozoospermia (OAT) is the most prevalent male infertility condition that is mainly caused by sperm DNA fragmentation (SDF). This study compared the sensitivity and effectiveness of two different approaches for analyzing SDF in patients with OAT: sperm chromatin dispersion (SCD) and terminal deoxynucleotidyl transferase dUTP nick end labeling (TUNEL). MATERIALS AND

methodsIn this study, which received ethical committee approval, participants were divided in to normal and OAT groups (n=20 for each). both TUNEL and SCD assays were used to analyze the sperm DNA fragmentation. And Malondialdehyde (MDA) levels was measured to determine levels of lipid peroxidation in the seminal plasma.

resultsThe TUNEL assay showed better ability to predict OAT patients than that of the SCD. For our patient population, the projected cut-off points for the DNA fragmentation index of 29% and 19% were reported using the TUNEL and SCD tests, respectively. Seminal levels of MDA were significantly higher in the OAT group (P=0.002) than that of control group.

conclusionOAT patients showed higher MDA levels of seminal plasma and DNA fragmentation than the control group. Although sperm DNA fragmentation can be detected with high efficiency and sensitivity using both TUNEL and SCD assays, the TUNEL test was found to be a more accurate predictor for OAT patients.

Indexed as

DNA FragmentationligoasthenoteratozoospermiaSensitivitySpecificitySperm Chromatin DispersionTerminal Deoxynucleotidyl Transferase dUTP Nick End Labeling

Identifiers

PMID42038129
PMCPMC11826392

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