Evidence map›Paper›PMID 42037763›Full record

ArticleBio-protocol2026

Quantitative Analysis of Splenic Natural Killer Cells of Mice Using Imaging Flow Cytometry.

Mohammad N Amin, Md Saqline Mostaq, Mohammad B Uddin, Yong-Yu Liu

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Bio-protocol, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

4 authors.

Mohammad N AminSchool of Basic Pharmaceutical and Toxicological Sciences, University of Louisiana at Monroe, Monroe, LA, USA.
Md Saqline MostaqSchool of Basic Pharmaceutical and Toxicological Sciences, University of Louisiana at Monroe, Monroe, LA, USA.
Mohammad B UddinSchool of Basic Pharmaceutical and Toxicological Sciences, University of Louisiana at Monroe, Monroe, LA, USA.
Yong-Yu LiuSchool of Basic Pharmaceutical and Toxicological Sciences, University of Louisiana at Monroe, Monroe, LA, USA.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Natural killer (NK) cells are crucial innate immune effectors, mediating cytotoxicity against cancer and infected cells through receptors such as NKG2D. Reliable quantification of NK cell subsets is essential for evaluating NK cell-based immune responses in cancer research. Unlike other assays, including traditional flow cytometry used in assessing NK cells, imaging flow cytometry (IFC) is a simple and direct method for quantitative analysis of NK cells. This protocol describes the necessary procedures, including harvesting splenocytes, acquiring these cells labeled with NKG2D antibodies, and analyzing IFC data with IDEAS

Indexed as

Analyzing IFC dataCancer cellsImaging flow cytometryImmune cytotoxicityNatural killer cells

Identifiers

PMID42037763
PMCPMC13103969

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.