Evidence map›Paper›PMID 42037378›Full record

ArticleMicrobiology spectrum2026

Reproducibility crisis in isothermal amplification: lessons from benchmarking LAMP assays.

Lena Piglmann, Lena Campostrini, Regina Sommer, Alexander K T Kirschner, Rudolf Krska, Andreas H Farnleitner, Claudia Kolm, Georg H Reischer

Abstract read
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Lena PiglmannInstitute of Chemical, Environmental and Bioscience Engineering, Working Area Molecular Diagnostics, IFA Tulln, TU Wien, Tulln, Austria.ORCID 0009-0001-6871-7652
Lena CampostriniICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria.ORCID 0000-0001-6602-6455
Regina SommerICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria.ORCID 0000-0002-7781-1915
Alexander K T KirschnerICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria.ORCID 0000-0002-9797-3073
Rudolf KrskaInstitute of Bioanalytics and Agro-Metabolomics, Department of Agricultural Sciences, IFA Tulln, University of Natural Resources and Life Sciences Vienna (BOKU), Tulln, Austria.ORCID 0000-0001-6843-9755
Andreas H FarnleitnerICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria.ORCID 0000-0002-0542-5425
Claudia KolmICC Interuniversity Cooperation Centre Water & Health, Vienna, Austria.ORCID 0000-0002-0174-8665
Georg H ReischerInstitute of Chemical, Environmental and Bioscience Engineering, Working Area Molecular Diagnostics, IFA Tulln, TU Wien, Tulln, Austria.ORCID 0000-0002-3962-8685

Funding

Austrian Research Promotion Agency 43502638Gesellschaft für Forschungsförderung Niederösterreich FTI21-D-012
6 · The paper itself

Abstract

The number of published isothermal amplification assays has increased substantially in recent years. Unfortunately, no harmonized guidelines, such as the Minimum Information for Publication of Quantitative Real-Time PCR Experiments guidelines, exist for publishing these methods, often resulting in incomplete reporting of assay composition and performance. In this study, we systematically evaluated nine published loop-mediated isothermal amplification (LAMP) assays for the detection of

Indexed as

Molecular Diagnostic TechniquesNucleic Acid Amplification TechniquesPseudomonas aeruginosaPseudomonas InfectionsBenchmarkingDNA, BacterialHumansLimit of DetectionReproducibility of ResultsSensitivity and SpecificityDNA, Bacterialassay characteristicsisothermal amplificationLAMPmolecular diagnosticsPseudomonas aeruginosareproducibility

Identifiers

PMID42037378
PMCPMC13228004

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.