ArticleAnalytical chemistry2026
Multiplex Detection and Quantification of miRNAs in Drug Delivery Systems Using a Signal-Off Electrochemical Platform.
Article in Analytical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Liquid biopsy in breast cancer: current biomarker platforms and clinical applications.Exploration of targeted anti-tumor therapy · 2026Review
Corrections and comments
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Authors and funding
9 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Lipid nanoparticles (LNPs) are widely used for the delivery of therapeutic microRNAs (miRNAs), especially in coformulated systems where multiple targets are combined to enhance therapeutic efficacy. However, the simultaneous quantification of miRNAs within LNP formulations remains a major analytical challenge for pharmaceutical quality control. This work presents a dual-working electrochemical biosensor for multiplexed, selective, and amplification-free quantification of two miRNAs (miR-4676 and miR-6503) encapsulated in LNPs. The biosensor was fabricated on a dual-working screen-printed graphite electrode via gold electrodeposition, followed by site-specific immobilization of methylene blue (MB)-labeled ss-DNA probes. After surfactant-mediated LNP lysis, square wave voltammetry (SWV) enabled hybridization-based signal-off detection of the released targets. The sensor showed excellent analytical performance, with limits of detection of 0.96 nM for miR-4676 and 0.98 nM for miR-6503. High selectivity was confirmed through cross-reactivity experiments. In real formulations with different miRNA ratios (1:1 and 2:1), the sensor achieved quantification accuracies of 88% and 84%, respectively, compared to a standard fluorimetric assay. Unlike conventional methods, the platform enabled individual miRNA quantification in complex mixtures. This study introduces an electrochemical platform enabling multiplexed miRNA quantification within LNPs, providing a rapid, low-cost, and portable solution for quality control of miRNA-based formulations.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.