ArticleWorld journal of microbiology & biotechnology2026
Comprehensive characterization and genome-resolved insights into the probiotic potential of Bacillus sp. KNSH39 isolated from Litopenaeus vannamei intestine.
Article in World journal of microbiology & biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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Who cites it
1 citing paper in PubMed.
- Antibiofilm, Antimicrobial, and Limited Ammonia-Reduction Activities of Bacillus cereus-Group Isolates From Hot Spring Sediment and Rubber Latex Serum for Aquaculture Water Management.Water environment research : a research publication of the Water Environment Federation · 2026Article
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Authors and funding
10 authors.
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Abstract
Probiotic Bacillus species are being investigated as sustainable interventions to enhance health and disease resilience in aquaculture. However, the functional basis, biosafety profile, and genomic determinants of probiotic suitability in shrimp gut-associated Bacillus strains remain insufficiently characterized. In this study, a Bacillus strain (KNSH39) isolated from the intestine of Pacific white shrimp (Litopenaeus vannamei) was evaluated using integrated phenotypic, functional, and genome-resolved approaches. Classical assays assessed morphology, sporulation, antibiotic susceptibility, gastrointestinal tolerance, storage stability, and antibacterial activity of cell-free supernatant under thermal and pH stress. Hybrid whole-genome sequencing using Oxford Nanopore Technologies and Illumina platforms enabled high-quality assembly, followed by comprehensive functional annotation, mobilome analysis, biosynthetic gene cluster prediction, and comparative genomics. KNSH39 exhibited strong sporulation capacity (98.04%), a non-hemolytic phenotype, high tolerance to acidic and bile conditions, and formulation-dependent storage stability, with gel-bead encapsulation markedly improving viability. Cell-free supernatant inhibited Streptococcus agalactiae and Aeromonas hydrophila in a concentration-, time-, temperature-, and pH-dependent manner, retaining activity after heating to 120 °C. Genome analysis revealed a 5.49 Mb Bacillus cereus–group genome with complete central metabolic pathways, extensive quorum sensing systems, diverse secondary metabolite biosynthetic gene clusters, and an open pangenome structure. Collectively, these findings suggest that KNSH39 strain presents as a promising aquaculture probiotic candidate, exhibiting thermally stable antibacterial potential and well-defined genomic attributes.
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