Evidence map›Paper›PMID 42036315›Full record

ArticleThe Journal of reproduction and development2026

Holding temperature to transport immature bovine oocytes in collection media critically affects their developmental competence.

Kenichiro Sakaguchi, Thomas Chia-Tang Ho, Moeka Saito, Takashi Tanida, Yojiro Yanagawa, Seiji Katagiri, Kohei Kawano

Abstract read
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In one paragraph

Article in The Journal of reproduction and development, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Kenichiro SakaguchiLaboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Thomas Chia-Tang HoLaboratory of Veterinary Theriogenology, Joint Department of Veterinary Medicine, Faculty of Applied Biological Sciences, Gifu University, Gifu 501-1193, Japan.
Moeka SaitoLaboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Takashi TanidaLaboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Yojiro YanagawaLaboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Seiji KatagiriLaboratory of Theriogenology, Department of Clinical Sciences, Faculty of Veterinary Medicine, Hokkaido University, Sapporo 060-0818, Japan.
Kohei KawanoLaboratory of Reproductive Physiology, Faculty of Environmental, Life, Natural Science and Technology, Okayama University, Okayama 700-8530, Japan.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

We investigated the holding condition in collection media to establish transportation system of immature bovine cumulus-oocyte complexes (COCs). We examined holding temperature (38°C or 20°C), basal media (Lactated Ringer's solution and TCM199) and supplementation of hypoxanthine, an inhibitor of meiotic resumption. As a result, no blastocyst was obtained from COCs held at 38°C for 6 h or longer, whereas blastocysts were obtained even after 24 h holding in all experimental groups held at 20°C. The blastulation rate was maintained (42%) in TCM199 with hypoxanthine group even after 12 h holding, while reduction of the blastulation rates were observed in all other groups (P < 0.05). After 24 h of storage, Lactated Ringer's solution without hypoxanthine showed the highest blastulation rate (40%). In conclusion, holding temperature of oocytes critically affects the developmental competence of oocytes, and oocytes held at 20°C maintain their developmental competence for at least 12 h.

Indexed as

In Vitro Oocyte Maturation TechniquesOocytesAnimalsBlastocystCattleCulture MediaEmbryonic DevelopmentFemaleFertilization in VitroTemperatureCulture MediaHolding temperatureImmature oocytesIn vitro production (IVP)Oocyte transportationOvum pick-up (OPU)

Identifiers

PMID42036315
PMCPMC13441542

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.