Evidence map›Paper›PMID 42033023›Full record

ArticleChembiochem : a European journal of chemical biology2026

Mechanistic Insight into Conformational Control of Enzyme Activity by Genetically Encoded Metal-Responsive Switches.

Payal, Jonathan Thirman, Katherine A Edmonds, Sandip Mishra, Nathan Blackwell, Yasmine S Zubi, Benoît Roux, Jared C Lewis

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Chembiochem : a European journal of chemical biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0citing papers in PubMed
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1 · What the graph read from it

What it found

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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

PayalDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0002-1097-3565
Jonathan ThirmanDepartment of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA.ORCID 0000-0002-0096-1763
Katherine A EdmondsDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0002-1282-9858
Sandip MishraDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID 0009-0001-3463-4605
Nathan BlackwellDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.
Yasmine S ZubiDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0001-7306-517X
Benoît RouxDepartment of Biochemistry and Molecular Biology, University of Chicago, Chicago, Illinois, USA.ORCID 0000-0002-5254-2712
Jared C LewisDepartment of Chemistry, Indiana University, Bloomington, Indiana, USA.ORCID 0000-0003-2800-8330

Funding

Graduate Training Program in Quantitative and Chemical Biology at Indiana University BloomingtonT32GM131994 · NIGMS · TRUSTEES OF INDIANA UNIVERSITY · PI JARED C LEWIS · 2019 to 2026
$2.4M
Army Research Laboratory W911NF-18-1-0200NIGMS NIH HHS T32 GM131994NIH HHS T32 GM131994
6 · The paper itself

Abstract

We previously introduced a genetically encoded, metal-responsive system for reversible control of protein function based on metal chelation by bipyridylalanine (BpyAla) residues. The efficacy of this linking group approach was demonstrated in two structurally and functionally distinct enzymes, Pyrococcus furiosus prolyl oligopeptidase (Pfu POP) and Photinus pyralis luciferase (Pluc). Here, we investigate the mechanistic basis of this switching in Pfu POP. Fluorescence-based metal competition assays and molecular dynamics (MD) simulations were conducted to quantify Ni(II) binding affinity and evaluate the structural response to Bpy

Indexed as

NickelPyrococcus furiosusSerine EndopeptidasesMolecular Dynamics SimulationProlyl OligopeptidasesProtein ConformationNickelProlyl OligopeptidasesSerine Endopeptidasesartificial metalloenzymeconformational switchingprolyl oligopeptidaseunnatural amino acid

Identifiers

PMID42033023
PMCPMC13109687

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.