Evidence map›Paper›PMID 42032457›Full record

ArticleBMC genomics2026

Single-cell transcriptomic and chromatin accessibility atlas of peripheral blood mononuclear cells reveals immune cell heterogeneity and breed-specific characteristics in Duroc and Meishan pigs.

Hairui Fan, Cui Du, Chao Xu, Haifei Wang, Yejun Wang, Zhengchang Wu, Bin Zhao, Chao Chen, Shaobin Shang, Ming-An Sun and 1 more

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Article in BMC genomics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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2 · The registry

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

11 authors.

Hairui Fan *Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Cui Du *Institute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Chao Xu *Key Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Haifei WangKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Yejun WangSchool of Basic Medicine, Shenzhen University Health Science Center, Shenzhen, Guangdong, 518060, China.
Zhengchang WuKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Bin ZhaoNational Clinical Research Center for Metabolic Diseases, Key Laboratory of Diabetes Immunology, Ministry of Education, and Department of Metabolism and Endocrinology, The Second Xiangya Hospital of Central South University, Changsha, Hunan, 410011, China.
Chao ChenDepartment of Pediatrics, Aflac Cancer and Blood Disorders Center, Winship Cancer Institute, Children's Healthcare of Atlanta, Emory University, Atlanta, GA, 30322, USA.
Shaobin ShangInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, China.
Ming-An SunInstitute of Comparative Medicine, College of Veterinary Medicine, Yangzhou University, Yangzhou, Jiangsu, 225009, China. mingansun@yzu.edu.cn.
Wenbin BaoKey Laboratory for Animal Genetics, Breeding, Reproduction and Molecular Design, College of Animal Science and Technology, Yangzhou University, Yangzhou, Jiangsu, 225009, China. wbbao@yzu.edu.cn.

Funding

National Key Research and Development Program of China 2023YFF1000900
6 · The paper itself

Abstract

backgroundPig is an important model for biomedical studies, yet the immune cell heterogeneity, the subtle relationship between immune cells and the inter-breed immune variation in pigs remains poorly characterized. Here, we generate single-cell transcriptomes for the Peripheral Blood Mononuclear Cells (PBMCs) of two pig breeds with distinct immune traits including Duroc and Meishan pigs – with the latter have better immune capacity.

resultsDozens of cell types are annotated, with γδ T lymphocytes making up one-fifth of all PBMCs. A list of putative novel markers is identified in each cell type, such as GATA3, SYTL3 and C9H1orf186 which are enriched in γδ T lymphocytes. The cellular composition and immune gene expression abundance are compared across breeds, showing that Meishan pigs have higher proportion of monocytes and their monocytes have enhanced expression of immune-related genes. Re-clustering analysis further identified the sub-populations of T, B and monocytes, with their inter-breed differences further characterized. We also generate single-cell chromatin maps for PBMCs of Duroc pigs and identify a list of transcription factors associated with each immune lineage. Comparison with human data demonstrates that while most cell populations and the corresponding markers match between pig and human, CD4+CD8+ DPTCs and γδ T cells are absent in human and one monocyte subset are absent in pigs.

conclusionsOverall, the present study examined the transcription levels and constructed the single-cell maps of blood immune cells between Duroc and Meishan pigs, which revealed the heterogeneity of immune cells in pigs and their differential characteristics among Duroc and Meishan pigs. This observation would be valuable for better understanding of the immune heterogeneity and immune traits in pigs.

Indexed as

ChromatinLeukocytes, MononuclearSingle-Cell AnalysisTranscriptomeAnimalsBreedingGene Expression ProfilingSingle-Cell Gene Expression AnalysisSwineChromatinImmune heterogeneityInter-breed immune variationPBMCScATAC-seqScRNA-seq

Identifiers

PMID42032457
PMCPMC13248445

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