Evidence map›Paper›PMID 42032408›Full record

ArticleBulletin of experimental biology and medicine2026

Effect of the Vesicular Fraction of Donor Semen Plasma on the Expression of AURKA and KLHL10 Genes in Cryopreserved Spermatozoa.

M Yu Gavrilov, N P Makarova, A P Sysoeva, V A Zhuchkov, E A Evtushenko, E E Bragina, V S Chernyshev, E A Kalinina

Abstract read
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Article in Bulletin of experimental biology and medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

M Yu GavrilovNational Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.
N P MakarovaNational Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia. np.makarova@gmail.com.
A P SysoevaNational Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.
V A ZhuchkovBranch of Shemyakin-Ovchinnikov Institute of Bioorganic Chemistry, Russian Academy of Sciences, Pushchino, Russia.
E A EvtushenkoLomonosov Moscow State University, Moscow, Russia.
E E BraginaA. N. Belozersky Institute of Physico-Chemical Biology, Lomonosov Moscow State University, Moscow, Russia.
V S ChernyshevNational Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.
E A KalininaNational Medical Research Center of Obstetrics, Gynecology, and Perinatology Named after Academician V. I. Kulakov, Ministry of Health of the Russian Federation, Moscow, Russia.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

The article investigates the influence of extracellular vesicles (EVs) isolated from donor seminal plasma (SP) on the expression of key reproductive genes AURKA and KLHL10 in human spermatozoa before and after cryopreservation. This study arises from the necessity to improve the effectiveness of assisted reproductive technologies (ART) and to advance the comprehension of how cryopreservation affects male gamete quality. A total of 17 sperm samples were examined. EVs were isolated from SP by asymmetric depth filtration and used for co-culturing with sperm samples prior to cryopreservation. Gene expression was analyzed by real-time PCR. The results demonstrated that sperm co-culturing with donor SP-EVs helped maintain or upregulate the expression of AURKA and KLHL10 genes that play critical roles in cell division and acrosome formation, respectively. These findings indicate a protective effect of EVs on spermatozoa during cryopreservation. The study highlights the potential utility of SP-EVs for augmenting sperm cryotolerance and boosting the efficacy of ART programs. Further research is warranted to investigate protein-level changes and functional implications of the observed gene expression patterns.

Indexed as

Aurora Kinase ACryopreservationSemenSpermatozoaCoculture TechniquesGene Expression RegulationHumansMaleSemen PreservationSperm ProteinsAurora Kinase ASperm Proteinsassisted reproductive technologies (ART)AURKA and KLHL10 gene expressioncryopreservationseminal plasmaspermatozoon

Identifiers

PMID42032408

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.