In one paragraphArticle in Nature communications, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
17 authors.
Markus JanetzkyDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0009-0001-3905-0019 Norman GeistDepartment of Biophysical Chemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0000-0001-9671-8882 Sabrina SchulzeDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0000-0002-0122-5688 Henriette RückertDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0009-0009-7817-5496 Kimani GatzemeyerDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Gottfried J PalmDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0000-0003-0329-0413 Leona BerndtDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Britta GirbardtDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Daniel WeisDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Ina MenyesDepartment of Biotechnology & Enzyme Catalysis, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Norma WelschDepartment of Pharmaceutical Biotechnology, University of Greifswald, Institute of Pharmacy, Greifswald, Germany.
Thomas SchwederDepartment of Pharmaceutical Biotechnology, University of Greifswald, Institute of Pharmacy, Greifswald, Germany.ORCID 0000-0002-7213-3596 Mark DörrDepartment of Biotechnology & Enzyme Catalysis, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0000-0003-3270-6895 Stefan KemnitzDepartment for High Performance Computing, University of Greifswald, University Computing Center, Greifswald, Germany.
Uwe T BornscheuerDepartment of Biotechnology & Enzyme Catalysis, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.ORCID 0000-0003-0685-2696 Mihaela DelceaDepartment of Biophysical Chemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany.
Michael LammersDepartment of Synthetic and Structural Biochemistry, University of Greifswald, Institute of Biochemistry, Greifswald, Germany. michael.lammers@uni-greifswald.de.ORCID 0000-0003-4168-4640 Funding
Deutsche Forschungsgemeinschaft (German Research Foundation) 441529220Deutsche Forschungsgemeinschaft (German Research Foundation) 445120888Deutsche Forschungsgemeinschaft (German Research Foundation) 534243417
6 · The paper itselfAbstract
Bacteria adjust their metabolism to the cellular energy state. AMP-forming acetyl-CoA-synthetase AcsA generates acetyl-CoA from acetate, ATP and CoA. In Bacilli, including Bacillus subtilis and Geobacillus stearothermophilus, AcsA is reversely transcribed upstream of the acu-operon encoding for the proteins AcuA, AcuB and AcuC. Lysine-acetyltransferase AcuA uses acetyl-CoA to acetylate and inactivate AcsA, while AcuC re-activates AcsA activity by deacetylation. How the counteracting activities of AcuA and AcuC are regulated is not understood. Here, we close this gap of knowledge and perform a structure-function analyzes on AcuB. These reveal AcuB forming a scissor-shaped dimer with each monomer consisting of an N-terminal Bateman domain binding to adenine nucleotides and a C-terminal ACT domain. Structural and biochemical studies as well as molecular dynamics simulations support that AMP bound AcuB binds and inhibits AcuC. Our data describe another layer of regulation of AcsA activity in Firmicutes coordinating acetate assimilation and dissimilation by the energy sensor AcuB.
Indexed as
Acetate-CoA LigaseAcetyl Coenzyme AAcetyltransferasesBacillus subtilisBacterial ProteinsGeobacillus stearothermophilusAdenosine MonophosphateMolecular Dynamics SimulationAcetate-CoA LigaseAcetyl Coenzyme AAcetyltransferasesAdenosine MonophosphateBacterial Proteins
Identifiers
PMID42031755
PMCPMC13121692
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