Evidence map›Paper›PMID 42030938›Full record

ArticleCell reports. Medicine2026

A longitudinal single-cell and spatial multiomic atlas of pediatric high-grade glioma.

Jonathan H Sussman, Derek A Oldridge, Wenbao Yu, Chia-Hui Chen, Abigail M Zellmer, Jiazhen Rong, Arianne Parvaresh-Rizi, Anusha Thadi, Austin Yang, Joseph S Tumulty and 22 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cell reports. Medicine, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 5 papers.

0numbers the graph read from it
0cells of the map it votes in
5citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

5 citing papers in PubMed.

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4 · The record

Corrections and comments

5 · Who and what money

Authors and funding

32 authors.

Jonathan H SussmanMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Graduate Group in Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Derek A OldridgeDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA; Center for Computational and Genomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Wenbao YuCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Chia-Hui ChenCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Abigail M ZellmerCenter for Computational and Genomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Jiazhen RongGraduate Group in Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Department of Statistics and Data Science, University of Pennsylvania, Philadelphia, PA, USA.
Arianne Parvaresh-RiziDepartment of Medicine, Warren Alpert Medical School of Brown University, Providence, RI, USA.
Anusha ThadiCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Austin YangCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Joseph S TumultyCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Barbara XiongMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Graduate Group in Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
David W WuMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Graduate Group in Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Yusha SunMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Neuroscience Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Shovik BandyopadhyayMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Cellular and Molecular Biology Graduate Group, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Jason XuMedical Scientist Training Program, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA; Graduate Group in Genomics and Computational Biology, Perelman School of Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Stephanie BrosiusCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Una Yamamoto BarkardottirCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Isabelle SekaCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Kyung Jin AhnCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Omar ElghawyDepartment of General Internal Medicine, University of Pennsylvania, Philadelphia, PA, USA.
Amy E BaxterCenter for Computational and Genomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Mateusz P KoptyraDepartment of Neurosurgery, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Rami S VanguriCenter for Computational and Genomic Medicine, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Stephanie McGroryCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Phillip B StormDepartment of Neurosurgery, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Nduka M AmankulorDepartment of Neurosurgery, Perelman School of Medicine, Philadelphia, PA, USA.
Mariarita SantiDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
Angela N ViaeneDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA.
Nancy ZhangDepartment of Statistics and Data Science, University of Pennsylvania, Philadelphia, PA, USA.
Thomas De RaedtDepartment of Pathology and Laboratory Medicine, Perelman School of Medicine at the University of Pennsylvania, Philadelphia, PA, USA; Center for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA.
Kristina ColeCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA.
Kai TanCenter for Childhood Cancer Research, Children's Hospital of Philadelphia, Philadelphia, PA, USA; Department of Pediatrics, University of Pennsylvania Perelman School of Medicine, Philadelphia, PA, USA; Center for Single Cell Biology, Children's Hospital of Philadelphia, Philadelphia, PA, USA. Electronic address: tank1@chop.edu.

Funding

Physician Scientist Training in Clinical OncologyK12CA076931 · NCI · UNIVERSITY OF PENNSYLVANIA · PI STEPHEN Patrick HUNGER, LYNN M. SCHUCHTER · 1997 to 2026
$16.5M
Data Analysis UnitU2CCA233285 · NCI · CHILDREN'S HOSP OF PHILADELPHIA · PI HUNGER, STEPHEN PATRICK, TAN, KAI · 2018 to 2023
$13.2M
NCI NIH HHS K12 CA076931NCI NIH HHS U2C CA233285
6 · The paper itself

Abstract

Pediatric high-grade glioma (pHGG) is an incurable central nervous system malignancy that is a leading cause of pediatric cancer death. While pHGG shares many similarities with adult glioma, it comprises distinct disease entities. In this study, we longitudinally profile a molecularly diverse cohort of 16 pHGG patients through single-nucleus RNA and ATAC sequencing, whole-genome sequencing, and CODEX spatial proteomics to capture the evolution of neoplastic and microenvironmental features during disease progression and treatment. We define a set of core pHGG neoplastic cell states and observe differential tumor-myeloid interactions between malignant cell phenotypes. We find that essential neuromodulators and the interferon response are upregulated post-therapy, implicating them as malignant cell-intrinsic targets. We observe an increase in oligodendrocytes upon progression and that they coordinate spatial motifs with proneural tumor cells. This multiomic atlas of longitudinal pHGG captures features of therapy response and provides a scalable reference for the study of pediatric brain tumors.

Indexed as

Brain NeoplasmsGliomaSingle-Cell AnalysisAdolescentChildChild, PreschoolFemaleGene Expression Regulation, NeoplasticHumansLongitudinal StudiesMaleMultiomicsNeoplasm GradingProteomicsSpatial TranscriptomicsTumor Microenvironmentcentral nervous system tumorsglioblastomapediatric high-grade gliomasingle-cell multiomicsspatial proteomicstranscriptional regulationtumor microenvironment

Identifiers

PMID42030938
PMCPMC13198283

What OpenQuestion holds

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LicenceCC BY-NC-ND
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.