ArticlePlanta2026
Genotype-specific optimization of in vitro regeneration and Agrobacterium-mediated transformation in indica rice with 35S:RUBY and CRISPR/LbCas12a system.
Article in Planta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
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conclusionThis study establishes a genotype-specific transformation system for indica rice cultivars Ranjit, Mahsuri, and Kon Joha using 35S:RUBY and CRISPR/LbCas12a constructs, enabling functional genomics studies and genetic improvement. The indica rice subspecies generally faces challenges in functional genomics and genetic improvement due to its recalcitrance to tissue culture and Agrobacterium-mediated transformation. Three indica rice varieties, Ranjit, Mahsuri, and Kon Joha, cultivated in Assam (India) were selected to optimize callus induction, regeneration, and genetic transformation. Ranjit and Mahsuri are high-yielding cultivars, whereas Kon Joha is an indigenous aromatic landrace of high commercial value. Initially, key steps, such as callus induction and regeneration, were optimized for Ranjit and Kon Joha using mature seed. Thereafter, for transformation, immature embryos were selected as explants because of their competence for agro-infection. The highest callus induction frequency of 70.33% and 90% was achieved with 3.0 and 3.5 mg L
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