Evidence map›Paper›PMID 42029774›Full record

ArticleArchives of microbiology2026

Control of anthracnose caused by Colletotrichum truncatum on dragon fruit (Hylocereus undatus) using recombinant chitinase.

Le Thi Ha Thanh, Nguyen Thi Thuy Tien, Dang Van Thanh, Nguyen Thi Yen Nhi, Nguyen Quang Duc Tien, Nguyen Hoang Loc

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Article in Archives of microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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3 · Its place in the literature

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0 citing papers in PubMed.

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4 · The record

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5 · Who and what money

Authors and funding

6 authors.

Le Thi Ha ThanhInstitute of Bioactive Compounds and Department of Biotechnology, University of Sciences, Hue University, Hue, 49000, Vietnam.
Nguyen Thi Thuy TienDepartment of Engineering and Food Technology, University of Agriculture and Forestry, Hue University, 49000, Hue, Vietnam.
Dang Van ThanhInstitute of Bioactive Compounds and Department of Biotechnology, University of Sciences, Hue University, Hue, 49000, Vietnam.
Nguyen Thi Yen NhiDepartment of Engineering and Food Technology, University of Agriculture and Forestry, Hue University, 49000, Hue, Vietnam.
Nguyen Quang Duc TienInstitute of Bioactive Compounds and Department of Biotechnology, University of Sciences, Hue University, Hue, 49000, Vietnam.
Nguyen Hoang LocInstitute of Bioactive Compounds and Department of Biotechnology, University of Sciences, Hue University, Hue, 49000, Vietnam. nhloc@hueuni.edu.vn.

Funding

Vingroup Innovation Foundation VINIF.2022.DA00117
6 · The paper itself

Abstract

Anthracnose, caused by Colletotrichum spp., is a major postharvest disease affecting dragon fruit (Hylocereus undatus) in Vietnam. In this study, a fungal strain was isolated from infected dragon fruits and identified based on morphological characteristics and multi-locus phylogenetic analysis (ITS region, and ACT, CHS-1, CAL, GAPDH, TUB2 genes) as C. truncatum DH31.1. The antifungal activity of a thermostable mutant chitinase I168L, derived from 42 kDa chitinase of Trichoderma asperellum SH16, was evaluated against C. truncatum. In vitro assays demonstrated that 60 U of I168L inhibited fungal growth, resulting in a colony diameter of 2.4 cm after 7 d, compared to 3.1 cm when treated with the same concentration of the wild-type chitinase. In vivo tests on dragon fruit showed that I168L effectively suppressed fungal growth at both 28 °C and 35 °C. Application of I168L with 100 U prevented C. truncatum DH31.1 infection for up to 4 d, with lesion diameters of only 0.2 cm after 7 d. Furthermore, curative application of I168L on post-infection dragon fruits reduced lesion expansion to 0.48 cm within 3 d. These findings highlight the potential of the thermostable mutant chitinase I168L as a promising biocontrol agent for postharvest disease management in dragon fruit.

Indexed as

CactaceaeChitinasesColletotrichumPlant DiseasesAntifungal AgentsFruitFungal ProteinsHypocrealesPhylogenyRecombinant ProteinsVietnamAntifungal AgentsChitinasesFungal ProteinsRecombinant ProteinsBiocontrol agentcross-infectionpostharvest disease managementTrichoderma asperellum

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.