ReviewBiophotonics discovery2025
High-speed fluorescence lifetime imaging microscopy: techniques, applications, and prospects.
Review in Biophotonics discovery, 2025. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 6 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
6 citing papers in PubMed.
- Superconducting nanowire single-photon detectors for enhanced biomedical imaging.Journal of biomedical optics · 2026Article
- Article
- Single-pixel imaging flow cytometry for biomedical research.Inflammation and regeneration · 2025Review
- Article
- Light and metabolism: label-free optical imaging of metabolic activities in biological systems [Invited].Biomedical optics express · 2025Review
- AlliGator: Open Source Fluorescence Lifetime Imaging Analysis in G.bioRxiv : the preprint server for biology · 2025Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
Significance: Fluorescence lifetime imaging microscopy (FLIM) has been gaining increasing attention due to its capability to provide robust, accurate, and quantitative measurements compared with conventional intensity-based fluorescence microscopy. However, the slow imaging speed of FLIM has long been a major limitation for expanding its applications in biology and medicine. Aim: We aim to discuss recent advancements in FLIM to enhance imaging speed and explore its biomedical applications and future prospects. Approach: We discuss high-speed FLIM techniques by categorizing them into time-domain and frequency-domain approaches, as well as wide-field and beam-scanning imaging schemes, with a focus on their combinations. Results: Recent advances in high-speed FLIM have been primarily driven by innovations in fluorescence detection schemes for wide-field imaging, as well as fluorescence excitation strategies for beam-scanning imaging. By enhancing FLIM imaging speed to levels comparable with intensity-based fluorescence microscopy, the observation of fast cellular dynamics, such as neural spiking, and large-scale image-based analysis of heterogeneous cells have become feasible. In addition, promising directions for the future development of high-speed FLIM include further utilization of fit-free analysis, implementation of video-rate 3D FLIM, and realization of FLIM-based cell sorting. Conclusions: The development of high-speed FLIM promises new opportunities in cell biology, biophysics, and neurology.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.