ArticleBMC plant biology2026
A multi-branched EMS mutant of Isodon lophanthoides var. graciliflorus exhibits significant differences in phytohormones and diterpenoids.
Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
Abstract
backgroundIsodon lophanthoides var. graciliflorus (Benth.) H. Hara, serves as one of the botanical sources for the traditional Chinese medicine "Xihuangcao". Mixed germplasm resources and insufficient high-quality cultivars hinder its sustainable and high-quality development.
resultsIn this study, we generated a multi-branched mutant B88, induced by ethyl methanesulfonate (EMS). It exhibits a 36.78% increase in branching, and a 39.64% increase in plant height at maturity compared to the wild-type (WT) plants, implying high-yield potential. Although Simple Sequence Repeat (SSR) molecular markers revealed genetic variations, it retained the same chromosome number as the WT, indicating genetic stability and continuity. Transcriptomics revealed differentially expressed genes (DEGs) enriched in phytohormone biosynthesis and signal transduction pathways, and metabolomic identified 54 differentially accumulated metabolites (DAMs), mainly flavonoids and terpenoids. DEGs and DAMs were significantly enriched in diterpenoid biosynthesis, particularly the gibberellin (GA) pathway, with 4.79-fold higher GA content compared to the WT and three key genes (ent-Copalyl Diphosphate Synthase, CPS; Gibberellin 20-oxidase, GA20ox; Gibberellin 2-oxidase, GA2ox) showing significant differences, suggesting that GA may have a comprehensive regulatory effect on plant height, branching, and accumulation of bioactive compounds in I. lophanthoides var. graciliflorus.
conclusionsThis study strongly supported the value of the breeding material-B88 mutant, enriched the genetic diversity of I. lophanthoides var. graciliflorus, and revealed the potential of EMS mutagenesis in the improvement of medicinal plants, offering new insights for elucidating the effects of EMS mutagenesis on the growth regulation, quality improvement, and germplasm innovation of I. lophanthoides var. graciliflorus.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.