Evidence map›Paper›PMID 42026474›Full record

ArticleBMC plant biology2026

Genome-wide identification and expression analysis of the ARF gene family in wax gourd (Benincasa hispida).

Xiaoxin Cheng, Piaoyun Sun, Jinqiang Yan, Xuling Zhai, Wenrui Liu, Dian Li, Jiesheng Hong, Zhenqiang Cao, Biao Jiang

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PubMed Full text at PMC Publisher
In one paragraph

Article in BMC plant biology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

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1citing papers in PubMed
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1 · What the graph read from it

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3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Review
4 · The record

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5 · Who and what money

Authors and funding

9 authors.

Xiaoxin Cheng *Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Piaoyun Sun *Guangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Jinqiang YanGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Xuling ZhaiGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Wenrui LiuGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Dian LiGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Jiesheng HongGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Zhenqiang CaoGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China.
Biao JiangGuangdong Key Laboratory for New Technology Research of Vegetables, Vegetable Research Institute, Guangdong Academy of Agricultural Sciences, Guangzhou, 510640, China. jiangbiao@gdaas.cn.

Funding

2024 Guangdong Rural Revitalization Strategy Special Project 2024-440440-103010206-0002Science and Technology Program of Guangdong 2024A1515012539, 2023A0505090005
6 · The paper itself

Abstract

backgroundAuxin Response Factors (ARFs) are key transcription factors regulating auxin-mediated plant growth and development. However, their roles in wax gourd (Benincasa hispida) remain unexplored.

resultWe identified 26 BhARF genes genome-wide, more than in cucumber, likely reflecting lineage-specific gene family expansion and species-specific evolutionary history. Phylogenetic analysis grouped them into seven groups, indicating evolutionary conservation. Motif and gene structure analyses showed high sequence similarity, while synteny analysis suggested lineage-specific expansion via dispersed duplication. Chromosomal mapping revealed uneven gene distribution. Comparative synteny indicated stronger conservation within Cucurbitaceae than with Arabidopsis. Transcriptome profiling showed BhARF6a and BhARF6b were highly expressed in fruit. Both localized to the nucleus, and transgenic Arabidopsis expressing BhARF6a and BhARF6b exhibited enhanced plant height and larger siliques; notably, BhARF6a significantly increased total seed weight, while BhARF6b mainly increased 1000-seed weight without significantly affecting total seed weight per plant. In addition, higher endogenous IAA levels were observed in the higher-yielding transgenic line.

conclusionOur study reveals the evolutionary patterns and functional significance of BhARF genes in wax gourd, especially BhARF6a and BhARF6b in fruit development. These findings offer a basis for future research and genetic improvement in Cucurbitaceae.

Indexed as

CucurbitaceaePlant ProteinsTranscription FactorsArabidopsisChromosome MappingGene Expression ProfilingGene Expression Regulation, PlantGenes, PlantGenome, PlantIndoleacetic AcidsMultigene FamilyPhylogenyPlants, Genetically ModifiedSyntenyIndoleacetic AcidsPlant ProteinsTranscription FactorsARF gene familyFruit developmentGenome-wide identificationWax gourd

Identifiers

PMID42026474
PMCPMC13285429

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