Evidence map›Paper›PMID 42024230›Full record

ArticleJournal of separation science2026

An Accurate Method Based on Ultra-High-Performance Liquid Chromatography-Electrospray Ionization-Tandem Mass Spectrometry for the Quantification of Five Cannabinoids in Juvenile and Adult Caenorhabditis elegans.

Estatira Sepehr, Yang Zhao, Cory A Vaught, Jessica A Camacho, Bonnie Welch, Jeffrey Yourick, Robert L Sprando, Piper Reid Hunt

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Journal of separation science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Estatira SepehrDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Yang ZhaoDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Cory A VaughtDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Jessica A CamachoDivision of Food Contact Substances, Human Foods Program, U. S. Food and Drug Administration (FDA), College Park, Maryland, USA.
Bonnie WelchDivision of Food and Environmental Safety, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Jeffrey YourickDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Robert L SprandoDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.
Piper Reid HuntDivision of Toxicology, Human Foods Program, U. S. Food and Drug Administration (FDA), Laurel, Maryland, USA.

Funding

Intramural FDA HHS FD999999
6 · The paper itself

Abstract

Caenorhabditis elegans is an alternative model organism for toxicology research that aligns with the 3Rs principle (Replace, Reduce, Refine) and new approaches to modernize toxicity testing strategies. This study developed and single-laboratory validated a sensitive ultra-high-performance liquid chromatography-electrospray ionization-tandem mass spectrometry (UHPLC-ESI-MS/MS) method for quantifying five cannabinoids- cannabidivarin, cannabigerol, cannabidiol, cannabinol, and cannabichromene- in juvenile and adult C. elegans matrices. Homogenized C. elegans samples were spiked with labeled internal standards and subjected to protein precipitation. The resulting supernatants were injected onto a Waters ACQUITY UPLC BEH C18 column (130 Å, 1.7 µm, 2.1 × 100 mm), coupled to an Agilent 6460 Triple Quadrupole mass spectrometer detector, allowing for the detection and quantification of target analytes in a total run time of 14 min using a 50 µL sample volume. Single-laboratory method validation was performed using spiked quality control samples, consisting of six replicates at four concentrations of all cannabinoids in juvenile and adult C. elegans matrices, analyzed over three consecutive days. The validated method demonstrated linear regression calibration curves with R

Indexed as

Caenorhabditis elegansCannabinoidsAnimalsChromatography, High Pressure LiquidLiquid Chromatography-Mass SpectrometrySpectrometry, Mass, Electrospray IonizationTandem Mass SpectrometryCannabinoidsCaenorhabditis eleganscannabinoidsmethod developmentsingle‐laboratory validationUHPLC‐ESI‐MS/MS

Identifiers

PMID42024230
PMCPMC13274484

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.