Evidence map›Paper›PMID 42023863›Full record

ArticleMicrobiology spectrum2026

A colloidal gold immunochromatographic test strip based on McAbs anti-S protein to detect porcine epidemic diarrhea virus.

Chaofan Liu, Wei Wang, Feiyan Wang, Jialu Hou, Qingbo Shi, Qinye Song, Chen Yuan

Abstract readEvaluation Study
PubMed Full text at PMC Publisher
In one paragraph

Article in Microbiology spectrum, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

7 authors.

Chaofan Liu *College of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.
Wei Wang *College of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.
Feiyan WangCollege of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.
Jialu HouCollege of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.
Qingbo ShiHanzhong Animal Disease Prevention and Control Center, Hanzhong, Shanxi, China.
Qinye SongCollege of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.ORCID 0000-0002-2899-8517
Chen YuanCollege of Veterinary Medicine, Hebei Agricultural University, Veterinary Biological Technology Innovation Center of Hebei Province, Baoding, China.ORCID 0009-0003-4727-2863

Funding

Hebei Provincial Department of Science and Technology 226Z6601GMajor Science and Technology Program of Shijiazhuang City 241500417ANational Natural Science Foundation of China 32202843
6 · The paper itself

Abstract

Porcine epidemic diarrhea (PED) is a highly contagious intestinal disease caused by the porcine epidemic diarrhea virus (PEDV), resulting in considerable economic losses for the global swine industry. Rapid diagnosis of PEDV is essential to develop effective prevention and control measures. The PEDV S1 protein serves as an efficient target for PEDV diagnosis. In this study, we successfully produced three monoclonal antibodies (McAbs) specific against the PEDV S1 protein, namely, H6, G3, and F2. ELISA showed that McAb F2 was IgG2a, McAbs H6 and G3 were IgG1, and the antigen site of McAb F2 was different from those of the other two antibodies. A colloidal gold immunochromatographic strip for PEDV detection was developed. McAb F2 conjugated with colloidal gold served as the detector antibody, McAb H6 was used as the capture antibody at the test line, and goat anti-mouse IgG was applied at the control line. The strip can specifically detect PEDV antigen without cross-reacting with TGEV, PCV2, PRV, PRRSV, or CSFV. It demonstrated high sensitivity with a detection limit of 1.2 TCID

Indexed as

Chromatography, AffinityCoronavirus InfectionsGold ColloidPorcine epidemic diarrhea virusSpike Glycoprotein, CoronavirusSwine DiseasesAnimalsAntibodies, MonoclonalAntibodies, ViralEnzyme-Linked Immunosorbent AssayMiceRapid Diagnostic TestsReagent StripsSensitivity and SpecificitySwineAntibodies, MonoclonalAntibodies, ViralGold ColloidReagent StripsSpike Glycoprotein, Coronaviruscolloidal gold immunochromatographic test stripmonoclonal antibodyPEDVS1

Identifiers

PMID42023863
PMCPMC13228074

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.