Background: Malaria remains a significant public health challenge in Sub-Saharan Africa, including Ghana. Children and adults present with varying clinical and biochemical pictures in response to Method: The cross-sectional study was conducted in a public and a private health facility in the Eastern Region of Ghana from August 2023 to September 2023. Participants of the study comprised children under 5 years and adults 18 years and above. Structured questionnaires were used to gather participants' demographic information and medical history. Blood samples of participants were analyzed with standard microscopy technique for Results: The study enrolled 362 participants, comprising of 71.8% (260/362) adults and 69.3% (251/362) females. No significant differences were observed among gender and age categories across parasitemia levels, although a higher proportion of children < 5 years (51.9%, 53/102) recorded high parasitemia. Adult participants with high parasitemia recorded significantly lower Hb, RBC, MCV, MCH and PLT but higher ALT and AST than those with lower and moderate parasitemia. However, there was no significant variations in urea and creatinine levels with regard to degree of parasitemia among the participants. Conclusion: High