Evidence map›Paper›PMID 42021874›Full record

ArticleSichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition2026

[Analysis of the Advantages and Clinical Validation of Real-Time Fluorescence Nucleic Acid Isothermal Amplification Detection Technology in Detecting Low Viral Load Samples From HBV-Infected Patients].

Qunfang Huang, Rubing Xie, Yanping Lan, Zhen Xun, Qishui Ou, Can Liu

Abstract readValidation StudyEnglish Abstract
PubMed Full text at PMC Publisher
In one paragraph

Article in Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical science edition, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Qunfang Huang// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.ORCID 0009-0007-4318-8233
Rubing Xie// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.
Yanping Lan// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.
Zhen Xun// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.
Qishui Ou// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.
Can Liu// （ 350005）Department of Laboratory Medicine, Fujian Key Laboratory of Laboratory Medicine, Gene Diagnosis Research Center, Fujian Clinical Research Center for Clinical Immunology Laboratory Test, The First Affiliated Hospital, Fujian Medical University, Fuzhou 350005, China.ORCID 0009-0001-9382-8899

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Objective: This paper examines the advantages and clinical application value of simultaneous amplification and testing (SAT) real-time fluorescent nucleic acid isothermal amplification detection technology for detecting low viral load samples in individuals infected with hepatitis B virus (HBV). Methods: Using reverse transcription real-time fluorescence quantitative PCR (RT-qPCR) as the reference, the detection performance of real-time fluorescence nucleic acid isothermal amplification detection technology (SAT) was evaluated, including linear range, precision, and detection limit. Both methods were used to detect samples, which were the national standard substances of hepatitis B virus ribonucleic acid (HBV RNA). A total of 170 patients with chronic HBV infection were included for methodological comparison. They were divided into a high-level group (serum HBV DNA > 100 IU/mL, Results: Compared with the RT-qPCR method for detecting HBV RNA, the SAT method demonstrated a wider linear range (10 Conclusion: The SAT method offers higher sensitivity and stability in detecting low-level HBV RNA. HBV RNA can serve as a serological marker for evaluating viral transcription activity and has clinical application value in managing patients who are HBV DNA-negative or have low HBV DNA levels.

Indexed as

Hepatitis B, ChronicHepatitis B virusNucleic Acid Amplification TechniquesReal-Time Polymerase Chain ReactionViral LoadAdultDNA, ViralFemaleHumansMaleMiddle AgedRNA, ViralSensitivity and SpecificityDNA, ViralRNA, ViralClinical trialCovalently closed circular DNA (cccDNA)Hepatitis B virusSimultaneous amplification and testingUltra-low limit detection

Identifiers

PMID42021874
PMCPMC13095794

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.