Evidence map›Paper›PMID 42021295›Full record

ArticleBMC oral health2026

The effects of hydrogen peroxide on the proliferation ability and odontogenic differentiation ability of hDPCs.

Ziqin Lin, Xiaohan Lin, Lu Yu, Tie Zhu, Jiaqing Qi, Jiayi Shen, Hua Wu, Yewen Zhu, Runche Zhang, Junjie Lin and 1 more

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In one paragraph

Article in BMC oral health, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

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Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

11 authors.

Ziqin Lin *School of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Xiaohan Lin *School of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Lu Yu *Stomatology Department, Haining People's Hospital, Haining, Zhejiang, 314400, China.
Tie ZhuSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Jiaqing QiSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Jiayi ShenSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Hua WuSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Yewen ZhuSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Runche ZhangSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Junjie LinSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China.
Rui HeSchool of Stomatology, Hangzhou Normal University, Hangzhou, Zhejiang, 311121, China. heruiminli@sina.com.

Funding

Hangzhou Medical and Health Technology Project ZD20240016
6 · The paper itself

Abstract

backgroundHydrogen peroxide (HP) is widely used in tooth whitening; however, the incidence of dentin hypersensitivity after tooth whitening is relatively high. This study aimed to evaluate the effect of different concentrations of HP bleaching solution on the viability of human dental pulp cells (hDPCs) and their odontogenic differentiation ability.

methodsThis study was divided into control group (no treatment) and experimental groups. The experimental groups were cultured with gradient HP concentration for 24 h, then the proliferation (through CCK-8), mineralization ability of hDPCs (through Alkaline Phosphatase activity assay and alizarin staining assay) and the expression level of odontogenic related gene (through real time PCR) were evaluated.

resultsCCK-8 method showed that, after 24 h cultured with HP, 1 µg/mL and 5 µg/mL groups had no significant difference compared with the control group while other groups all had inhibitory effects on the proliferation of hDPCs. Alkaline Phosphatase (ALP) activity assay and alizarin red staining assay showed that there was no significant difference between the 1 µg/mL group and the control group in the mineralization ability. After cultured for 7 days, 5 µg/mL and 10 µg/mL groups could increase the activity of ALP and promote the formation of mineralized nodules. The expression level of odontogenic related gene of 5 µg/mL group increased, while that of 10 µg/mL group decreased.

conclusionhDPCs can overcome the damage of HP and undergo a complete repair with stimulation of low concentration of HP (1 µg/mL, 5 µg/mL), whereas its odontogenic differentiation ability and cell homeostasis may be damaged under the stimulation of high concentration of HP (10 µg/mL).

Indexed as

Cell DifferentiationCell ProliferationDental PulpHydrogen PeroxideOdontogenesisTooth Bleaching AgentsAlkaline PhosphataseCells, CulturedCell SurvivalHumansReal-Time Polymerase Chain ReactionAlkaline PhosphataseHydrogen PeroxideTooth Bleaching AgentsCell proliferationHuman dental pulp cellsHydrogen peroxideOdontogenic differentiation

Identifiers

PMID42021295
PMCPMC13273938

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.