ArticleMethods in molecular biology (Clifton, N.J.)2026
Culture of Human Retinal Explants as an Ex Vivo Model for Retinal Gene Therapy.
Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
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Authors and funding
4 authors.
Funding
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Abstract
Optogenetic gene therapy may employ recombinant adeno-associated virus (AAV) vectors for specific preclinical applications in the treatment of inherited retinal diseases. Human retinal explants as an ex vivo model assist determine cellular tropism and the level of subsequent gene expression followed by delivery of viral vectors. Extracts of retinal tissue can be obtained and cultured for a certain time; thus, it is possible to transduce and confirm the effectiveness of the virus using various methods, including immunohistochemical staining. Consequently, we described method for harvesting and culturing human retinal fragments from divergent parts of the retina that differ anatomically and molecularly. The fragments were fixed, sectioned, and stained. A dissociation method has also been described to obtain a retinal cell suspension that can be used in other experiments such as flow cytometry or mRNA/protein level expression assays.
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Registered trials
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