Evidence map›Paper›PMID 42020903›Full record

ArticleMethods in molecular biology (Clifton, N.J.)2026

Ex Vivo Culture and Formalin-Fixed Paraffin Embedding of Murine Retinal Explants.

Susan Haller, Rebecca M Marton, Eliah R Shamir

Abstract read
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In one paragraph

Article in Methods in molecular biology (Clifton, N.J.), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors.

Susan HallerDepartment of Research Pathology, Genentech, Inc., South San Francisco, CA, USA.
Rebecca M MartonDepartment of Regenerative Medicine, Genentech, Inc., South San Francisco, CA, USA.
Eliah R ShamirDepartment of Research Pathology, Genentech, Inc., South San Francisco, CA, USA. shamire@gene.com.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Traumatic, inherited, and age-related degenerative diseases of the retina are characterized by irreversible loss of retinal neurons, and no present treatments are available to restore them. Cultured murine neuroretinal explants serve as a model of retinal injury and offer a high-throughput, ex vivo approach to test various therapeutic interventions. Here, we describe techniques to isolate and culture retinal explants and treat them with molecules and viruses to enable identification of novel therapeutic strategies. In order to assess the effectiveness of a potential intervention, we also describe protocols to handle friable retinal explants and prepare formalin-fixed paraffin-embedded sections for hematoxylin and eosin staining and immunofluorescence. We also provide tips to overcome common challenges in explant isolation, culture, embedding, and sectioning.

Indexed as

Paraffin EmbeddingRetinaTissue Culture TechniquesTissue FixationAnimalsFluorescent Antibody TechniqueFormaldehydeMiceStaining and LabelingFormaldehydeEx vivo cultureFormalin-fixed paraffin embeddingHematoxylin and eosin stainingHistogelImmunofluorescenceRetinaRetinal explant

Identifiers

PMID42020903

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.