Evidence map›Paper›PMID 42020736›Full record

ArticleNature2026

Quadruple pegRNA enables programmable and efficient large genomic insertion.

Ya-Jing Shi, Zi-Yi Ding, Ying Wu, Zhou He, Yi-Zhou Zhang, Yu-Lu Zhang, Yu-Ming Zhang, Xiang-Rui Huang, Hao Yin, Ying Zhang

Abstract read
PubMed Publisher
In one paragraph

Article in Nature, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers, 1 of them a synthesis that pooled it.

0numbers the graph read from it
0cells of the map it votes in
14citing papers in PubMed, 1 pooled it
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

14 citing papers in PubMed, 1 synthesis or guideline pooled it.

  1. Pooled it
  2. Article
  3. Gene editing using large insertions.Nature biotechnology · 2026
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ya-Jing Shi *State Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Zi-Yi Ding *State Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Ying Wu *State Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Zhou HeTaiKang Centre for Life and Medical Sciences, School of Artificial Intelligence, RNA Institute, Hubei Key Laboratory of Cell Homeostasis, Wuhan University, Wuhan, China.
Yi-Zhou ZhangState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.ORCID http://orcid.org/0009-0004-0976-1305
Yu-Lu ZhangState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Yu-Ming ZhangState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Xiang-Rui HuangState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China.
Hao YinState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China. haoyin@whu.edu.cn.ORCID http://orcid.org/0000-0001-9654-5985
Ying ZhangState Key Laboratory of Virology and Biosafety, Departments of Rheumatology and Immunology, Clinical Laboratory and Urology, Medical Research Institute, Frontier Science Center for Immunology and Metabolism, Zhongnan Hospital of Wuhan University, Wuhan University, Wuhan, China. ying.zhang84@whu.edu.cn.ORCID http://orcid.org/0000-0003-4044-0230

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Precise, site-specific insertion of large gene sequences holds great promise for the treatment of diverse genetic disorders. Although prime editing using paired guide RNAs (pegRNAs) can mediate targeted integration, insertion efficiency drops sharply for payloads exceeding 300 base pairs

Indexed as

Gene EditingGenomicsMutagenesis, InsertionalRNA, Guide, CRISPR-Cas SystemsAnimalsDNAHumansTransposasesDNARNA, Guide, CRISPR-Cas SystemsTransposases

Identifiers

PMID42020736

What OpenQuestion holds

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Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.