A New Type of Nonsuppressible Viremia Produced by HIV-Infected Macrophage.

backgroundHIV-1 RNA typically declines rapidly after initiation of antiretroviral therapy (ART), often reaching undetectable levels within a few weeks and remaining undetectable by standard assays. However, some patients receiving ART have persistent nonsuppressible viremia (NSV) that does not respond to treatment optimization or intensification. NSV can emerge at the time of ART initiation (primary NSV) or after being ART suppressed (secondary NSV). Here, we examine mechanisms producing primary NSV in 4 people undergoing ART.

methodsBlood samples were collected from 4 participants with advanced immunodeficiency who, despite being adherent to ART, required approximately a year or more to become virologically suppressed. Viral RNA and proviral DNA genomes were sequenced to examine HIV drug resistance, genome intactness, and genetic diversity. The ability of HIV Envs to facilitate efficient entry into cells expressing low levels of CD4, a proxy for macrophage tropism, was also assessed.

resultsBefore ART, blood contained HIV RNA genomes that were adapted to replication in CD4+ T cells and rapidly decayed after ART initiation. During ART, blood contained HIV genomes that were drug sensitive, genetically diverse, and macrophage tropic; moreover, they were not evolving and often had vpr defects.

conclusionsOur results suggest that in individuals with primary NSV, ART stopped virus replication, but large pools of long-lived HIV-infected macrophage continued to produce virus. This is mechanistically distinct from secondary NSV produced by CD4+ T-cell clones. Defects in vpr independently accumulated in macrophage-tropic lineages found in 3 participants, suggesting that vpr may affect survival or virus production from HIV-infected macrophage.