ArticlePloS one2026
Beyond Clavulanic Acid biosynthesis: Exploring the broad regulatory impact of BldD in Streptomyces clavuligerus ATCC 27064.
Article in PloS one, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
3 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
BldD is a global regulator of morphological development in Streptomyces, usually acting as a repressor of aerial mycelium formation. However, its role in metabolism and secondary biosynthesis in the wild-type strain Streptomyces clavuligerus remains poorly understood. In this study, we constructed a S. clavuligerus strain overexpressing bldD and compared it with the empty vector control strain during fermentation in soy protein isolate medium. For this, transcriptomic analyses were performed at 24 h and 72 h. We observed that BldD overexpression reduced CA production and repressed genes involved in cephamycin C biosynthesis, including lat and the pathway regulator ccaR. In contrast, genes associated with terpene biosynthesis, phage tail-like particles (CIS), and aerial mycelium formation (bldN, bldM, chaplins) were upregulated. These changes suggest that BldD may directly and indirectly redirects metabolic flux away from β-lactam biosynthesis while promoting morphological differentiation, alternative defense systems and a potential link with GlcNAc metabolism. By repressing β-lactam pathways and enhancing CIS and terpene gene expression, BldD shifts cellular priorities toward morphological development and ecological defense, underscoring species-specific differences from the well-studied model Streptomyces coelicolor. These results greatly contribute to the understanding of the regulatory mechanisms involved in CA biosynthesis.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.