Evidence map›Paper›PMID 42018442›Full record

ArticleSTAR protocols2026

Protocol for the genome-wide identification of intrinsic transcription factor binding motifs by mammalian-optimized pull-down sequencing.

Xuan Jiang, Wenxiang Zhang

Abstract read
In one paragraph

Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

2 authors.

Xuan JiangShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China.
Wenxiang ZhangShanghai Institute of Immunology, Department of Immunology and Microbiology, Shanghai Jiao Tong University School of Medicine, Shanghai 200025, China. Electronic address: wenxiang.zhang@sjtu.edu.cn.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Chromatin immunoprecipitation sequencing (ChIP-seq) maps in vivo transcription factor (TF) occupancy under native chromatin conditions. Here, we present a protocol for genome-wide profiling of intrinsic TF motifs using protein-DNA pull-down sequencing (PD-seq), a complementary in vitro technique based on DNA affinity purification sequencing (DAP-seq) with optimizations for mammalian systems. We describe steps for TF purification, naked genomic DNA preparation, protein-DNA pull-down, and sequencing-based analysis. Applied to FOXP3, PD-seq identifies T

Indexed as

Chromatin Immunoprecipitation SequencingTranscription FactorsAnimalsBinding SitesChromatin ImmunoprecipitationDNAHumansNucleotide MotifsProtein BindingDNATranscription FactorsChIPseqImmunologyMolecular Biology

Identifiers

PMID42018442
PMCPMC13123352

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.