ArticlePharmacological reports : PR2026
A validated FACS-based protocol for the isolation of neuronal and non-neuronal cells from frozen adult brain, suitable for integrated gene and protein analysis.
Article in Pharmacological reports : PR, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundThe isolation of intact cell populations from archived tissue is critical for understanding cell-type-specific functions in neuroscience. This study presents a streamlined, bench-ready fluorescence-activated cell sorting (FACS) protocol for the isolation of neuronal and non-neuronal populations from frozen adult rat cortex, specifically optimized for integrated RNA and protein analyses.
methodsThe method employs a gentle enzymatic digestion and mechanical trituration, followed by fixation with zinc salts, which successfully addresses the challenges of dissociating adult brain tissue while preserving key cellular markers. The protocol’s versatility is demonstrated by its compatibility with two distinct collection buffers, enabling the simultaneous preparation of samples for both RNA and protein analysis, performed by qRT-PCR and mass spectrometry, respectively.
resultsNeuronal (PE+) and non-neuronal (PE-) fractions were isolated with high purity, as confirmed by significant enrichment of Rbfox3, Map2, and Tubb3 (neuronal) and Gfap, Iba1, Olig2, and Cldn5 (non-neuronal) via qRT-PCR. Subsequent proteomic analysis further confirmed the distinct identities of the sorted populations, with key neuronal and non-neuronal markers showing significant enrichment in their respective fractions.
conclusionsDespite a conservative yield (ca. 20,000 neurons per sample) prioritized for cellular intactness, the obtained material was sufficient for high-sensitivity qPCR (mRNA/miRNA) and mass spectrometry. This accessible and time-efficient protocol provides a robust platform for parallel RNA and protein analysis of defined brain cell populations from archived frozen tissue.
Indexed as
Identifiers
42018106What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.