ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026
Localizable Fluorescent Metal Ion Indicators With Tunable Colors.
Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
4 authors.
Funding
Abstract
Elucidating the role of metal ion homeostasis in physiological and pathological processes requires detection tools with high sensitivity and selectivity, spectral versatility, and precise subcellular localization. Here, we introduce a modular strategy for generating fluorescent metal ion indicators that are comprised of a sulfonamide-functionalized chelator, a rhodamine derivative, and a ligand for bioconjugation to self-labeling proteins. With a concise three-step synthesis, the design allows tuning of both the emission color and ligand specificity of the resulting indicators. Specifically, we developed a family of bright, color-tunable potassium indicators that can be selectively coupled to intra- or extracellular HaloTag and SNAP-tag fusion proteins. The increase in fluorescence upon binding to HaloTag or SNAP-tag enabled wash-free live-cell imaging. The localized potassium indicators enabled the detection of dynamic potassium efflux in rat hippocampal neurons upon glutamate stimulation. Our work thus establishes a versatile platform for the generation of localizable fluorescent metal ion indicators and opens up new avenues for live-cell potassium sensing.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.