ArticleApplied and environmental microbiology2026
Bio-Rad and QIAGEN digital PCR platforms provide equivalent quantification for wastewater-based SARS-CoV-2 surveillance.
Thomas Clerkin, Steph Smith, Kevin Zhu, Denene Blackwood, Javier Gallard-Góngora, Drew Capone, Joe Brown, Rachel T Noble
Abstract read
In one paragraphArticle in Applied and environmental microbiology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
8 authors.
Thomas Clerkin *Department of Earth, Marine, and Environmental Sciences, Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, USA.ORCID 0000-0001-5239-8266 Steph Smith *Department of Earth, Marine, and Environmental Sciences, Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, USA.ORCID 0000-0002-3709-4464 Kevin ZhuDepartment of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.
Denene BlackwoodDepartment of Earth, Marine, and Environmental Sciences, Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, USA.
Javier Gallard-GóngoraDepartment of Earth, Marine, and Environmental Sciences, Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, USA.
Drew CaponeDepartment of Environmental and Occupational Health, School of Public Health-Bloomington, Indiana University Bloomington, Bloomington, Indiana, USA.ORCID 0000-0002-2138-6382 Joe BrownDepartment of Environmental Sciences and Engineering, Gillings School of Global Public Health, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina, USA.ORCID 0000-0002-5200-4148 Rachel T NobleDepartment of Earth, Marine, and Environmental Sciences, Institute of Marine Sciences, University of North Carolina at Chapel Hill, Morehead City, North Carolina, USA.ORCID 0000-0001-9071-8312 Funding
UNC-CH CENTER FOR ENVIRONMENTAL HEALTH &SUSCEPTIBILITYP30ES010126 · NIEHS · UNIV OF NORTH CAROLINA CHAPEL HILL · PI Hazel B Nichols · 2001 to 2026
$36.3MNIEHS NIH HHS P30 ES010126UNC | UNC-CH | North Carolina Policy Collaboratory Tracking SARS-CoV-2 in the Wastewater Across a Range of North Carolina Municipalities
6 · The paper itselfAbstract
Digital PCR (dPCR) is increasingly used for wastewater surveillance due to its precision, absolute quantification, and reduced sensitivity to inhibition compared to quantitative PCR (qPCR). Although the Bio-Rad QX200 and QIAGEN QIAcuity dPCR platforms are widely adopted, their performance has not been directly compared for wastewater applications. We conducted a blinded comparison of these platforms using 93 archived wastewater influent samples from North Carolina collected in 2021-2022, spanning three orders of magnitude in SARS-CoV-2 concentration (1 × 10
Indexed as
COVID-19Polymerase Chain ReactionSARS-CoV-2WastewaterAnimalsHumansNorth CarolinaSensitivity and SpecificityWastewater-Based Epidemiological MonitoringWastewaterCDC NWSSdigital PCRdroplet digital PCRmethod validationplatform comparisonpublic health surveillancequantitative detectionSARS-CoV-2wastewater-based epidemiologywastewater surveillance
Identifiers
PMID42017669
PMCPMC13188893
What OpenQuestion holds
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