Identification and functional analysis of NAD

Background: Pulmonary hypertension (PH) is a severe progressive disease characterised by elevated pulmonary vascular resistance and right ventricular hypertrophy. Increasing evidence has highlighted the vital role of nicotinamide adenine dinucleotide (NAD Methods: Using the Gene Expression Omnibus database and Limma R package, we identified differentially expressed genes (DEGs) of PH and downloaded NMRGs from Kyoto Encyclopedia of Genes and Genomes and Reactome databases. Candidate NMRGs were subsequently identified by overlapping DEGs, NMRGs, and module genes obtained by weighted gene co-expression network analysis. The diagnostic value of these candidate NMRGs was evaluated using receiver operator characteristic (ROC) curve analysis, and gene set enrichment analysis (GSEA) was performed to explore the functional roles of hub genes. CIBERSORT algorithm was employed to assess immune cell infiltration in the PH microenvironment. Finally, the functional role of target genes in PH was validated through Results: Through comprehensive bioinformatics analyses across multiple datasets, we identified two NMRGs: NT5E and CD38. ROC analysis confirmed the higher predictive accuracy of NT5E, with area under the ROC curve values reaching 0.891 and 0.894 in GSE113439 and GSE53408 datasets, respectively. GSEA revealed that patients with high NT5E expression exhibited significant enrichment of PH-related biological functions and pathways. Given the relationship between NAD Conclusion: Based on bioinformatics analysis and