Evidence map›Paper›PMID 42016306›Full record

ArticleiScience2026

Integrated targeted sequencing reveals unique tissue-of-origin and donor-derived cell-free DNA signatures in stable organ transplant recipients.

Nicholas Kueng, Fanny Sandberg, Daniel Sidler, Vanessa Banz, Annalisa Berzigotti, Charlotte K Y Ng, Carlo R Largiadèr, Ursula Amstutz

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in iScience, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Donor-Derived Cell-Free DNA in Pancreas-Kidney, Heart-Kidney, and Liver-Kidney Multiorgan Transplant Recipients (MOTR).Transplant international : official journal of the European Society for Organ Transplantation · 2025
    Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

8 authors.

Nicholas KuengDepartment of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Fanny SandbergDepartment of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Daniel SidlerDepartment of Nephrology and Hypertension, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Vanessa BanzDepartment of Visceral Surgery and Medicine, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Annalisa BerzigottiDepartment of Hepatology, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Charlotte K Y NgIRCCS Humanitas Research Hospital, Milan, Italy.
Carlo R LargiadèrDepartment of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.
Ursula AmstutzDepartment of Clinical Chemistry, Inselspital, Bern University Hospital, University of Bern, Bern, Switzerland.

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Solid organ transplantation is a life-saving treatment for end-stage organ disease, yet current methods to assess graft health rely on invasive biopsies. We applied an integrated targeted deep sequencing assay to simultaneously quantify donor-derived and tissue-of-origin (TOO) cell-free DNA (cfDNA) in plasma from liver and kidney transplant recipients and healthy individuals. The assay sensitively detected cell-type-specific cfDNA and revealed that cfDNA composition differed markedly between stable transplant recipients and healthy individuals. Early after transplantation, dynamic cfDNA changes were observed reflecting tissue-specific injury and recovery processes unique to each organ type. These findings show the potential of combining donor-derived and TOO cfDNA analysis to provide a more comprehensive view of transplant recipient health and possibly improve non-invasive monitoring of both graft-related and systemic complications.

Indexed as

Biological sciencesImmunologyprecision medicine

Identifiers

PMID42016306
PMCPMC13092851

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.