ArticleMicrobial biotechnology2026
Genetically Boosting Electron Transfer in Electroactive Biofilms for Improved Sensitivity of Microbial Fuel Cell-Based Biosensing.
Article in Microbial biotechnology, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Electroactive biofilms (EABs) hold significant potential for applications in bioenergy, biosensing and wastewater treatment; however, their performance is frequently hindered by inefficient extracellular electron transfer (EET). A notable example of this limitation is the significant impact of low EET efficiency on the sensitivity of EAB-based biosensors. To address this, we engineered Geobacter sulfurreducens anode biofilms by enhancing intracellular levels of cyclic GMP-AMP (cGAMP) through the overexpression of cGAMP synthase. This approach resulted in the formation of thin yet highly conductive biofilms. When utilized in a microbial fuel cell biosensor, the engineered biofilm demonstrated a 20-fold improvement in detection limit for Cd(II), achieving 0.03 mg L
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