Evidence map›Paper›PMID 42014878›Full record

ArticleNature methods2026

NirFAP680: a highly bright and stable large Stokes shift near-infrared fluorogen-activating protein.

Zhengda Chen, Li Jiang, Xiaoyu Liu, Yajie Shi, Yang Liu, Ling Jiang, Jiaxin Li, Xinyi Huang, Siyu Zhou, Bingkun Bao and 11 more

Abstract read
PubMed Publisher
In one paragraph

Article in Nature methods, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 4 papers.

0numbers the graph read from it
0cells of the map it votes in
4citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

4 citing papers in PubMed.

  1. Non-Covalent Reversibly Photoconvertible Fluorescent Tags for Wash-Free Protein Labeling.Advanced science (Weinheim, Baden-Wurttemberg, Germany) · 2026
    Article
  2. Article
  3. Bright monomeric fluorescent protein elite-niRFP704 for two-channel near-infrared STED nanoscopy.Proceedings of the National Academy of Sciences of the United States of America · 2026
    Article
  4. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

21 authors.

Zhengda Chen *Optogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.ORCID http://orcid.org/0009-0008-5828-0099
Li Jiang *School of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Xiaoyu LiuOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Yajie ShiOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Yang LiuOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Ling JiangOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Jiaxin LiSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Xinyi HuangSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.
Siyu ZhouOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Bingkun BaoSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.ORCID http://orcid.org/0009-0008-2710-8843
Bibi ZhangOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Qiuning LinSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China.ORCID http://orcid.org/0000-0002-9418-9590
Qi BianOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Yuxin YinOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Fangting ZuoOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.ORCID http://orcid.org/0000-0001-6668-9427
Ni SuFrontiers Medical Center, Tianfu Jincheng Laboratory, Chengdu, China.
Yun TangOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.
Yuzheng ZhaoOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China.ORCID http://orcid.org/0000-0001-5400-0135
Yi YangOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China. yiyang@ecust.edu.cn.ORCID http://orcid.org/0000-0001-7896-1184
Xianjun ChenOptogenetics & Synthetic Biology Interdisciplinary Research Center, Shanghai Frontiers Science Center of Optogenetic Techniques for Cell Metabolism, School of Pharmacy, East China University of Science and Technology, Shanghai, China. xianjunchen@ecust.edu.cn.ORCID http://orcid.org/0000-0002-8475-332X
Linyong ZhuSchool of Biomedical Engineering, Shanghai Jiao Tong University, Shanghai, China. linyongzhu@sjtu.edu.cn.ORCID http://orcid.org/0000-0002-0398-7213

Funding

National Natural Science Foundation of China (National Science Foundation of China) 21937004National Natural Science Foundation of China (National Science Foundation of China) 32121005National Natural Science Foundation of China (National Science Foundation of China) 32150028National Natural Science Foundation of China (National Science Foundation of China) 91857202
6 · The paper itself

Abstract

Near-infrared (NIR) genetically encoded fluorescent tags are superior for new imaging capabilities ranging from multiplexed imaging to deep-tissue imaging. Here, we describe the development of NirFAP680; NirFAP680 is an NIR fluorogen-activating protein (FAP) that consists of a newly designed NIR fluorogen termed HBMT and an engineered protein tag termed NirFAP. NirFAP680 has an order of magnitude greater cellular brightness and superior photostability compared to currently available NIR FAPs and fluorescent proteins in both single- and two-photon excitation and allows robust imaging of proteins in live cells and in vivo. Owing to its unique spectroscopic property of a >100 nm Stokes shift, NirFAP680 can also serve as a superb receptor for fluorescence or bioluminescence resonance energy transfer, allowing real-time monitoring of protein-protein interactions in live cells and sensitive bioluminescence imaging in vivo, respectively. Therefore, NirFAP680 will likely be useful for advanced biological imaging in live cells and in vivo.

Indexed as

Fluorescent DyesLuminescent ProteinsAnimalsFluorescence Resonance Energy TransferHumansMiceSpectroscopy, Near-InfraredFluorescent DyesLuminescent Proteins

Identifiers

PMID42014878

What OpenQuestion holds

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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.