ArticleChemphyschem : a European journal of chemical physics and physical chemistry2026
A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.
Article in Chemphyschem : a European journal of chemical physics and physical chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
1 citing paper in PubMed.
- A Versatile Tool to Predict and Guide RESOLFT Images Based on Photoswitching, Labelling and Optical Properties.Chemphyschem : a European journal of chemical physics and physical chemistry · 2026Article
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Authors and funding
5 authors.
Funding
Abstract
Reversibly switchable fluorescent proteins (RSFPs) transition many times between dark and fluorescent states under minimal light doses. The photoswitching can happen at different speed, contrast and length, and it is often challenging for users to select the optimal imaging scheme to generate images with high contrast and spatial resolution. Here, we experimentally investigate the photophysical properties of different RSFPs under imaging conditions, together with an in silico exploration of their role in nanoscale image formation. We developed open-source software that uses measured parameters such as brightness, switching speed, photoswitching fatigue, labelling densities, noise and illumination type to generate the related RESOLFT (reversible saturable/switchable optical fluorescence transition) super-resolution image. This tool can be used to select optimal imaging schemes for known RSFPs and to guide the rational development of new proteins.
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Registered trials
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