Evidence map›Paper›PMID 42012805›Full record

ArticleAnalytical sciences : the international journal of the Japan Society for Analytical Chemistry2026

Determination of okadaic acid in scallop using microfluidic-based fluorescence polarization immunoassay.

Shunsuke Chida, Kazuki Takahashi, Mao Fukuyama, Motohiro Kasuya, Ayuko Imai, Anatoly V Zherdev, Sergei A Eremin, Masatoshi Maeki, Akihiko Ishida, Hirofumi Tani and 3 more

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Article in Analytical sciences : the international journal of the Japan Society for Analytical Chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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0cells of the map it votes in
0citing papers in PubMed
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1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

0 citing papers in PubMed.

No citing paper in PubMed yet.

4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Shunsuke ChidaGraduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Kazuki TakahashiGraduate School of Chemical Sciences and Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Mao FukuyamaInstitute of Multidisciplinary Research for Advanced Materials, Tohoku University, 2-1-1, Katahira, Aoba-ku, Sendai, 980-8577, Japan.
Motohiro KasuyaFaculty of Production Systems Engineering and Sciences, Komatsu University, Nu 1-3, Shicho-machi, Komatsu, Ishikawa, 923-8511, Japan.
Ayuko ImaiTianma Japan, Ltd., Shinkawasaki Twin Tower West 28F, 1-1-2, Kashimada, Saiwai-ku, Kawasaki, 212-0058, Japan.
Anatoly V ZherdevA. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, Moscow, Russia, 119071.
Sergei A EreminA. N. Bach Institute of Biochemistry, Research Center of Biotechnology, Russian Academy of Sciences, Leninsky Prospect 33, Moscow, Russia, 119071.
Masatoshi MaekiDivision of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Akihiko IshidaDivision of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Hirofumi TaniDivision of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan.
Koji ShigemuraTianma Japan, Ltd., Shinkawasaki Twin Tower West 28F, 1-1-2, Kashimada, Saiwai-ku, Kawasaki, 212-0058, Japan.
Akihide HibaraDepartment of Chemistry, School of Science, Institute of Science Tokyo, 2-12-1 Ookayama, Meguro-ku, Tokyo, 152-8551, Japan.
Manabu TokeshiDivision of Applied Chemistry, Faculty of Engineering, Hokkaido University, Kita 13 Nishi 8, Kita-ku, Sapporo, 060-8628, Japan. tokeshi@eng.hokudai.ac.jp.ORCID http://orcid.org/0000-0002-4412-2144

Funding

Agriculture, Forestry and Fisheries Research Council JPJ008837Russian Science Foundation Project 20-43-07001
6 · The paper itself

Abstract

A microfluidic device-based fluorescence polarization immunoassay (FPIA) enabling rapid assay completion within 20-25 min was developed for the rapid screening of okadaic acid (OA) in shellfish. A fluorescent tracer was synthesized by conjugating OA to a fluorescent dye, and two anti-OA monoclonal antibodies (7E1 and 9G3) were evaluated to optimize assay performance. Binding activity tests revealed that the 9G3 antibody exhibited a lower dissociation constant and superior assay performance. Calibration curves were constructed, and the dynamic range and sensitivity of the assay were investigated, with particular emphasis on OA concentrations around the regulatory limit (0.16 mg/kg). The applicability of the developed FPIA to real samples was assessed through spike-recovery tests using scallop extracts. Although a slight decrease in sensitivity was observed due to the presence of methanol in the extracts, OA concentrations near the regulatory threshold could be reliably discriminated. These results demonstrate that the developed microfluidic FPIA provides a rapid and simple screening approach for determining whether OA concentrations in shellfish exceed established regulatory limits, thereby supporting routine monitoring and on-site analysis.

Indexed as

Lab-On-A-Chip DevicesMicrofluidic Analytical TechniquesOkadaic AcidPectinidaeAnimalsFluorescence Polarization ImmunoassayShellfishOkadaic Acidfluorescence polarization immunoassaymicrofluidic deviceokadaic acidshelfish poison

Identifiers

PMID42012805

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.