Evidence map›Paper›PMID 42012551›Full record

ArticleMikrochimica acta2026

A label-free fluorescence/colorimetric dual-mode sensor for ultrasensitive detecting Staphylococcus aureus through the alkaline phosphatase activity.

Huihui Su, Yi Fan, Fangzhou Lv, Kaixin Liu, Li Fu, Li Jia

Abstract read
PubMed Publisher
In one paragraph

Article in Mikrochimica acta, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

6 authors.

Huihui SuMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
Yi FanMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
Fangzhou LvMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
Kaixin LiuMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.
Li FuMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China. lee940414lee@126.com.
Li JiaMinistry of Education Key Laboratory of Laser Life Science & Guangdong Provincial Key Laboratory of Laser Life Science & Guangzhou Key Laboratory of Spectral Analysis and Functional Probes, College of Biophotonics, School of Optoelectronic Science and Engineering, South China Normal University, Guangzhou, 510631, China.

Funding

National Natural Science Foundation of China 21675056
6 · The paper itself

Abstract

Rapid and accurate detection of Staphylococcus aureus (S. aureus) is significant to ensure food safety and curb the spread of the epidemic. Conventional single-mode optical sensors for detecting S. aureus are susceptible to interference from complex sample matrices. We propose a label-free fluorescence/colorimetric dual-mode sensor for S. aureus through its intrinsic alkaline phosphatase (ALP) activity. The biosensor is based on carbon dot-loaded manganese dioxide nanosheets (CDs@MnO2) nanocomposites. In the presence of S. aureus, ALP catalyzed the hydrolysis of ascorbic acid 2-phosphate magnesium ester, yielding ascorbic acid. The resulting ascorbic acid suppresses the sensor’s oxidase-like activity and mitigates the inner filter effect, thereby reducing the absorbance of substrate and restoring fluorescence. This method achieved a low detection limit of 2 cfu mL‒1 and was successfully applied to milk, milk powder, pork, and lettuce samples, yielding recoveries of 90.7%‒110% with relative standard deviations below 5.9%.

Indexed as

Alkaline PhosphataseBiosensing TechniquesColorimetryStaphylococcus aureusAnimalsAscorbic AcidCarbon Quantum DotsFood MicrobiologyLimit of DetectionManganese CompoundsMilkNanocompositesOxidesSpectrometry, FluorescenceAlkaline PhosphataseAscorbic AcidManganese Compoundsmanganese dioxideOxidesAlkaline phosphataseFluorescence/colorimetric dual-mode sensorFood analysisMnO2 nanozymeOxidase-like activityStaphylococcus aureus

Identifiers

PMID42012551

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.