Evidence map›Paper›PMID 42010781›Full record

ArticlePest management science2026

A field-deployable RPA-CRISPR/Cas12a dual-mode assay for rapid detection of Fusarium oxysporum in Nicotiana tabacum.

Ke Yue, Xiaoyan Liang, Yaoqiang Wang, Ning Jiang, Yanxia Hu, Jiaen Su, Canhua Lu, Hongbo Gao, Xiaotong Gai, Wei Guo

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PubMed Full text at PMC Publisher
In one paragraph

Article in Pest management science, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

10 authors.

Ke Yue *Yunnan Academy of Tobacco Agricultural Sciences, Kunming, P. R. China.
Xiaoyan Liang *Institute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, P. R. China.
Yaoqiang WangInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, P. R. China.
Ning JiangYunnan Academy of Tobacco Agricultural Sciences, Kunming, P. R. China.
Yanxia HuDali Tobacco Company of Yunnan, Dali, P. R. China.
Jiaen SuDali Tobacco Company of Yunnan, Dali, P. R. China.
Canhua LuYunnan Academy of Tobacco Agricultural Sciences, Kunming, P. R. China.
Hongbo GaoDali Tobacco Company of Yunnan, Dali, P. R. China.
Xiaotong GaiYunnan Academy of Tobacco Agricultural Sciences, Kunming, P. R. China.
Wei GuoInstitute of Food Science and Technology, Chinese Academy of Agricultural Sciences/Key Laboratory of Agro-products Quality and Safety Control in Storage and Transport Process, Ministry of Agriculture and Rural Affairs, Beijing, P. R. China.ORCID https://orcid.org/0000-0002-6411-6227

Funding

China National Tobacco Corporation (CNTC) 110202401006(JY-06)Yunnan Academy of Tobacco Agricultural Sciences 2024530000241006Yunnan Applied Fundamental Research Projects 202405AD350100
6 · The paper itself

Abstract

backgroundFusarium oxysporum is a devastating soil-borne pathogen that causes severe economic losses in tobacco and other crops, necessitating rapid and accurate detection methods for effective disease management. Herein, we developed a dual-mode RPA-Cas12a platform incorporating both lateral flow dipstick (LFD) and fluorescence-based detection (FBD), targeting the CYP51C gene to enable rapid and equipment-facilitated detection of F. oxysporum under isothermal conditions.

resultsThe entire assay from sample to result can be completed within 68 min. The platform offers two complementary detection formats. The LFD mode provides visual qualitative results with a detection limit of 360 copies, making it ideally suited for rapid on-site screening. In contrast, the FBD mode achieves a detection limit of 3.6 copies, demonstrating 100-fold higher sensitivity and enabling precise quantitative analysis.

conclusionThe assay successfully identified F. oxysporum in both artificially inoculated and field-collected tobacco samples, showing high concordance with fluorescence intensity. This work provides a sensitive, rapid, and practical diagnostic tool for the on-site detection and monitoring of tobacco root rot caused by F. oxysporum. © 2026 Society of Chemical Industry.

Indexed as

CRISPR-Cas SystemsFusariumNicotianaNucleic Acid Amplification TechniquesPlant DiseasesRapid Diagnostic TestsCRISPR/Cas12aFusarium oxysporumrapid detectionrecombinase polymerase amplification (RPA)tobacco root rot

Identifiers

PMID42010781
PMCPMC13352207

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.