Evidence map›Paper›PMID 42009821›Full record

ArticleScientific reports2026

Transcriptome analysis and effects of low salinity stress on ovarian development in Litopenaeus vannamei.

Qingyun Liu, Min Peng, Chunling Yang, Yuliu Huang, Weilin Zhu, Qiangyong Li, Xiuli Chen, Huanling Wang, Hong Liu, Digang Zeng and 1 more

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PubMed Full text at PMC Publisher
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Article in Scientific reports, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.

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5 · Who and what money

Authors and funding

11 authors.

Qingyun LiuHuazhong Agricultural University, Wuhan, China.
Min PengGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Chunling YangGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Yuliu HuangGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Weilin ZhuGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Qiangyong LiGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Xiuli ChenGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China.
Huanling WangHuazhong Agricultural University, Wuhan, China.
Hong LiuHuazhong Agricultural University, Wuhan, China.
Digang ZengGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China. zengdigang@126.com.
Yongzhen ZhaoGuangxi Academy of Fishery Sciences, Guangxi Key Laboratory of Aquatic Genetic Breeding and Healthy Aquaculture, Nanning, 530021, China. yongzhenzhao@hotmail.com.

Funding

Guangxi Natural Science Foundation 2025GXNSFAA069905Guangxi Science and Technology Major Project Guike AA23062046Post Scientist Project CARS-48-02Research on Key Technologies for Intelligent Breeding and Ecological Aquaculture of Superior and Characteristic Aquatic Species in Guangxi GUIKE-LT2600640018
6 · The paper itself

Abstract

Litopenaeus vannamei is an economically important shrimp species that can survive in a variety of environments; however, low salinity compromises its reproduction. To investigate whether salinity affects ovarian development in L. vannamei, we reared female shrimp under optimal (30‰) and low (25‰, 20‰, 15‰) salinity conditions. On day 26 post-induction, the ovarian maturation rates were 67.7%, 58.3%, 56.7%, and 31.7% for the 30‰, 25‰, 20‰, and 15‰ salinity groups, indicating that low-salinity compromises ovarian maturation. To provide mechanistic insight, we performed comparative transcriptomic analysis of hepatopancreas, eyestalk, gill, and ovary tissues five days post-maturation induction. GO and KEGG enrichment analyses revealed that upregulated differentially expressed genes (DEGs) under low-salinity conditions (n = 2359 total) were primarily associated with immune response, apoptosis regulation, polysaccharide metabolism, and antioxidation pathways, reflecting stress adaptation to maintain cellular homeostasis. Downregulated DEGs (n = 2008 total) were significantly enriched in pathways related to energy metabolism, lipid metabolism, and estrogen signaling, indicating the inhibition of metabolic and reproductive pathways. Trend analysis identified the ovarian development-related gene, vitelline membrane outer layer protein 1 (VMO1), whose expression was verified to decrease significantly with declining salinity. These results provide practical guidance for L. vannamei breeding and a new perspective for understanding salinity-mediated reproductive regulation.

Indexed as

OvaryPenaeidaeSalt StressTranscriptomeAnimalsFemaleGene Expression ProfilingSalinityLitopenaeus vannameiOvarian developmentSalinity stressTranscriptome analysis

Identifiers

PMID42009821
PMCPMC13250162

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