ArticleeLife2026
The robust, high-throughput, and temporally regulated roxCre and loxCre reporting systems for genetic modifications in vivo.
Mengyang Shi, Jie Li, Xiuxiu Liu, Kuo Liu, Lingjuan He, Wenjuan Pu, Wendong Weng, Shaohua Zhang, Huan Zhao, Kathy Lui and 1 more
Abstract read
In one paragraphArticle in eLife, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
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4 · The recordCorrections and comments
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5 · Who and what moneyAuthors and funding
11 authors.
Mengyang Shi *CAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0009-0005-7805-5436 Jie Li *CAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0009-0002-5550-5148 Xiuxiu LiuCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Kuo LiuKey Laboratory of Systems Health Science of Zhejiang Province, School of Life Science, Hangzhou Institute for Advanced Study, University of Chinese Academy of Sciences, Hangzhou, China.ORCID https://orcid.org/0000-0003-0757-7833 Wenjuan PuCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.
Wendong WengCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0000-0003-3730-7965 Shaohua ZhangCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0000-0002-7300-4091 Huan ZhaoCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0000-0001-6319-949X Bin ZhouCAS CEMCS-CUHK Joint Laboratories, New Cornerstone Science Laboratory, State Key Laboratory of Cell Biology, CAS Center for Excellence in Molecular Cell Science, Shanghai Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences, University of Chinese Academy of Sciences, Shanghai, China.ORCID https://orcid.org/0000-0001-5278-5522 Funding
CAS Project for Young Scientists in Basic Research YSBR-012National Key Research & Development Program of China 2020YFA0803202National Key Research & Development Program of China 2022YFA1104200National Key Research & Development Program of China 2023YFA1800700National Key Research & Development Program of China 2023YFA1801300National Key Research & Development Program of China 2024YFA1803302National Natural Science Foundation of China 32100592National Natural Science Foundation of China 32100648National Natural Science Foundation of China 32370783National Natural Science Foundation of China 32370897National Natural Science Foundation of China 82088101New Cornerstone Science Foundation New Cornerstone Investigator ProgramResearch Funds of Hangzhou Institute for Advanced Study 2022ZZ01015Research Funds of Hangzhou Institute for Advanced Study B04006C01600515Shanghai Pilot Program for Basic Research-CAS, Shanghai Branch JCYJ-SHFY-2021-0
6 · The paper itselfAbstract
Cre-loxP technology, a cornerstone in fate mapping and in vivo gene function studies, faces challenges in achieving precise and efficient conditional mutagenesis through inducible systems. This study introduces two innovative genetic tools designed to overcome these limitations. The first, roxCre, enables DreER-mediated Cre release, paving the way for intersectional genetic manipulation that permits increased precision and efficiency. The second, loxCre, facilitates conditional gene targeting by allowing CreER lines to induce Cre expression with significantly enhanced efficiency. These tools incorporate a fluorescent reporter for genetic lineage tracing, simultaneously revealing efficient gene knockout in cells marked by the reporter. These strategies hold great potential for precise and efficient exploration of lineage-specific gene functions, marking a significant advancement in genetic research methodologies.
Indexed as
Gene TargetingIntegrasesAnimalsGenes, ReporterMiceCre recombinaseIntegrasesdevelopmental biologydual-recombinaseendothelial cellsgenetic manipulationgeneticsgenomicslivermouse
Identifiers
PMID42008290
PMCPMC13095210
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