ArticleCurrent protocols2026
Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research.
Article in Current protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
1 citing paper in PubMed.
- Laboratory Protocols for Candidatus Liberibacter asiaticus Transmission Biology and Management Research.Current protocols · 2026Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
6 authors.
Funding
Abstract
Candidatus Liberibacter asiaticus (CLas), the causal agent of Huanglongbing, is transmitted by the Asian citrus psyllid (Diaphorina citri) in a persistent, propagative manner. Reproducible transmission studies require not only controlled inoculation assays but also standardized propagation of citrus host plants, maintenance of D. citri colonies, and reliable pathogen detection. This study presents a comprehensive set of coordinated protocols that collectively support controlled CLas transmission experimentation. The workflow includes propagation of Citrus macrophylla for colony and assay use; generation and maintenance of CLas-infected citrus and D. citri colonies; synchronization of D. citri populations to enable CLas acquisition; and a controlled single-D. citri transmission assay with defined inoculation access periods, and a Dellaporta-based nucleic acid extraction method compatible with citrus and psyllid tissues. Quantitative PCR detection is integrated throughout to verify infection status, determine CLas titer, and monitor CLas movement and systemic colonization in citrus over time. By combining standardized plant production, vector infection systems, transmission assays, and molecular diagnostics, these coordinated methods provide a unified framework for testing hypotheses about vector competence, pathogen-vector interactions, microbial co-infections, host susceptibility, and environmental effects on transmission. Together, the procedures enable consistent and comparable assessment of CLas acquisition, dissemination, and transmission outcomes under controlled laboratory conditions. © 2026 The Author(s). Current Protocols published by Wiley Periodicals LLC. Basic Protocol 1: Controlled laboratory method for assessing Candidatus Liberibacter asiaticus transmission by Diaphorina citri Support Protocol 1: Generation of Candidatus Liberibacter asiaticus-infected Citrus macrophylla and Diaphorina citri Basic Protocol 2: Dellaporta total nucleic acid extraction Basic Protocol 3: Citrus macrophylla propagation.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.