Actinobacteria TFDB: An integrated view of transcription factors in Actinobacteria.

Actinobacteria represent a prolific source of bioactive natural products. However, the complex transcriptional regulatory networks in these bacteria, particularly the interplay between transcription factors (TFs) and their regulatory ligands (TF-RLs), remain poorly characterized and lack dedicated resources. In this context, we introduce the Actinobacteria Transcription Factor Database (Actinobacteria TFDB), a comprehensive repository that systematically integrates TF-centric data across 25 representative species. The current version encompasses 629 TFs, classified into 69 families, documents 11,776 TF-target relationships and 28 TF posttranslational modification sites. Uniquely, it features a dedicated collection of 54 experimentally validated TF-RL interactions. Beyond providing standardized annotations, sequence and structural features, and regulatory networks, Actinobacteria TFDB incorporates a specialized TF-RL module that enables interactive exploration and visualization of allosteric regulatory mechanisms. By consolidating multi-dimensional TF data from diverse sources, this resource empowers systems-level analyses and facilitates the rational design of regulatory strategies to activate silent biosynthetic gene clusters and optimize metabolite production. The database is publicly available at http://mingleadgene.com:9315/#/home.