ArticleACS omega2026
Substrate Stiffness Modulates Cell-Network Topology in Human-Derived Neurons.
Article in ACS omega, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
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Authors and funding
8 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Using confocal imaging and network topology analysis, we investigated how substrate stiffness affects adhesion and connectivity in hiPSC-derived neuronal cultures grown on polydimethylsiloxane (PDMS). We compared soft (∼12 kPa) and stiff (∼1.5 MPa) substrates with matched surface properties to isolate mechanical effects. Confocal analysis of NCAM expression revealed higher levels on soft PDMS at 10 DIV, indicating enhanced neuronal adhesion and outgrowth. Network topology analysis showed that only soft PDMS supported increased clustering, reduced path length, and higher small-worldness, reflecting more efficient connectivity. These results underscore the importance of substrate compliance in promoting neuronal development and inform scaffold design for neural engineering.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.