Evidence map›Paper›PMID 42003806›Full record

ArticleAdvanced science (Weinheim, Baden-Wurttemberg, Germany)2026

Allosteric DNAzyme-Enabled Sensitive and Multiplex Detection of Biomarkers for Rapid Diagnosis of Urinary Tract Infections.

Yanzhe Shen, Chenzhi Shi, Xiaowei Ma, Pengfei Hou, Haomin Zhang, Juanxiu Qin, Li Pan, Guilin Li, Lifei Gao, Qian Ma and 3 more

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Advanced science (Weinheim, Baden-Wurttemberg, Germany), 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it
0cells of the map it votes in
1citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed.

  1. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors.

Yanzhe ShenInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Chenzhi ShiInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Xiaowei MaInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Pengfei HouInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Haomin ZhangInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Juanxiu QinInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Li PanInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Guilin LiNational Eco & Tech Zone, Autobio Diagnostics Co., Ltd., Zhengzhou, Henan, China.
Lifei GaoNational Eco & Tech Zone, Autobio Diagnostics Co., Ltd., Zhengzhou, Henan, China.
Qian MaIntellinosis Biotechnology Co., Ltd., Shanghai, China.
Donglei YangInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Min LiInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.
Pengfei WangInstitute of Molecular Medicine, Department of Laboratory Medicine, Shanghai Key Laboratory for Nucleic Acid Chemistry and Nanomedicine, Renji Hospital, School of Medicine, Shanghai Jiao Tong University, Shanghai, China.ORCID https://orcid.org/0000-0002-3125-763X

Funding

National Key Research and Development Program of China 2021YFA0910100National Natural Science Foundation of China 22304112
6 · The paper itself

Abstract

Urinary tract infections (UTI) are among the most prevalent infectious diseases, demanding rapid diagnosis to provide timely therapeutic interventions. Urinary molecules can indicate the responses of the host cells to pathogenic infections, which may serve as markers for UTI diagnosis. Sensitive and multiplex detection of urinary markers in one-pot has remained challenging. Herein, we developed an allosteric DNAzyme-based biosensor, denoted as SMART (sensitive and multiplex detection of ATP and miRNAs for UTI diagnosis), to enable rapid UTI diagnosis. Multiple sensing modules were integrated into a unimolecular DNA strand of perfect stoichiometry and excellent thermodynamic stability that collectively led to enhanced detection capability. The binding of targets to the detection module induces conformational reconfiguration of SMART to activate sequence-specific catalytic cleavage against fluorescent RNA reporters. SMART demonstrated remarkable sensitivity (ATP: ∼pM; miRNAs: ∼fM) and multiplexing capability (∼4 markers) to realize extraction-free, preamplification-free, and rapid (∼2.5 hr) detection of UTI markers. SMART-based UTI diagnosis model yielded a detection accuracy of 95.5% in a cohort of 164 patients. SMART may serve as a technical platform for detecting various markers that could be applied to the diagnosis of UTI and many other diseases in the future.

Indexed as

BiomarkersBiosensing TechniquesDNA, CatalyticUrinary Tract InfectionsAdenosine TriphosphateHumansMicroRNAsSensitivity and SpecificityAdenosine TriphosphateBiomarkersDNA, CatalyticMicroRNAsbiomarkersclinical diagnosisDNAzymemultiplex detectionurinary tract infection

Identifiers

PMID42003806
PMCPMC13335575

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.