ArticleJournal of applied genetics2026
A novel engineered vector for TA cloning of PCR products.
Article in Journal of applied genetics, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionOptimized vectors are crucial in modern molecular biology because they greatly enhance the efficiency of the DNA cloning procedure. This study aimed to design a novel engineered vector containing an HphI cassette to enhance TA cloning efficiency.
methodsThe pTZ57R/T plasmid served as the basis for developing the novel Engineered vector (pTZ57R/T-2000). Three restriction sites (respectively, XbaI- HphI -SalI) were inserted into amplified fragments by specific primers. The PCR amplified fragments (1,000 bp) were digested by XbaI and SalI enzymes, then ligated into the modified pTZ57R/T plasmid (pTZ57R/T-HphI), which had been prepared by digestion with the same enzymes. Finally, the transformation and cloning efficiencies of the PCR products were assessed through the number of clones and colony PCR, respectively.
resultsThe statistical analyses showed a significant difference in transformation efficiency between pTZ57R/T-2000 and ddT-tailed vectors (P < 0.0001), with the pTZ57R/T-2000 vector producing an average of 54 clones compared to 26.67 clones for the ddT-tailed vectors. However, there was no significant difference in TA cloning efficiency between the two vectors (P = 0.1069). DISCUSSION: The success rate of pTZ57R/T-2000 vector transformation is higher than ddT-tailed vector in terms of the number of clones. However, the TA cloning efficiency is lower in pTZ57R/T-2000 vectors compared to ddT-tailed vectors, to eliminate the assumption of a shortage of genes for cloning. To enhance the pTZ57R/T-2000 efficiency, it is recommended to use pre-divided tubes to reduce T-head damage during defreezing and pipetting.
Indexed as
Identifiers
42002697What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.