ArticleThe Journal of biological chemistry2026
A direct fluorescence assay for quantitative analysis of ornithine decarboxylase activity and inhibitor screening.
Jae-Yeon Choi, Grzegorz Bereta, Matthew Furry, Christopher D Vanderwal, Przemysław Grudnik, Choukri Ben Mamoun
Abstract read
In one paragraphArticle in The Journal of biological chemistry, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
0numbers the graph read from it
0cells of the map it votes in
0citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
2 · The registryThe trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
3 · Its place in the literatureWho cites it
0 citing papers in PubMed.
No citing paper in PubMed yet.
4 · The recordCorrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
5 · Who and what moneyAuthors and funding
6 authors.
Jae-Yeon ChoiDepartment of Internal Medicine, Section of Infectious Diseases, New Haven, Connecticut, USA.
Grzegorz BeretaMałopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland.
Matthew FurryDepartment of Chemistry, University of California, Irvine, California, USA.
Christopher D VanderwalDepartment of Chemistry, University of California, Irvine, California, USA; Department of Pharmaceutical Sciences, University of California, Irvine, California, USA.
Przemysław GrudnikMałopolska Centre of Biotechnology, Jagiellonian University, Kraków, Poland.
Choukri Ben MamounDepartment of Internal Medicine, Section of Infectious Diseases, New Haven, Connecticut, USA; Department of Microbial Pathogenesis, New Haven, Connecticut, USA; Department of Pathology, Yale School of Medicine, New Haven, Connecticut, USA. Electronic address: choukri.benmamoun@yale.edu.
Funding
Fosinopril analogs for the treatment of human babesiosisR01AI153100 · NIAID · YALE UNIVERSITY · PI BEN MAMOUN, CHOUKRI · 2021 to 2025
$3.6MChemical Synthesis of Bioactive DiterpenoidsR35GM145252 · NIGMS · UNIVERSITY OF CALIFORNIA-IRVINE · PI Christopher D Vanderwal · 2022 to 2026
$2.1MAntigen Discovery and Vaccine Development for Human Babesia ParasitesR01AI152220 · NIAID · YALE UNIVERSITY · PI BEN MAMOUN, CHOUKRI · 2020 to 2024
$2.1MParasite-Derived Vesicles in Babesia virulence and Vaccine DevelopmentR56AI177660 · NIAID · YALE UNIVERSITY · PI BEN MAMOUN, CHOUKRI, KASHANCHI, FATAH · 2024 to 2024
$874kPANK Activators for the treatment of pantothenate kinase-associated neurodegenerationR43NS127740 · NINDS · VIRTUS THERAPEUTICS CORPORATION · PI REGAN, JESSICA · 2023 to 2024
$797kAntifungals targeting pantothenate phosphorylationR43AI176966 · NIAID · CURATIX, LLC · PI CHOI, JAE-YEON · 2023 to 2024
$594kA Point of Care Test for Babesia microtiR43AI188960 · NIAID · L2 DIAGNOSTICS, LLC · PI Alan Gordon Sutherland · 2025 to 2026
$568kNIAID NIH HHS R01 AI152220NIAID NIH HHS R01 AI153100NIAID NIH HHS R43 AI176966NIAID NIH HHS R43 AI188960NIAID NIH HHS R56 AI177660NIGMS NIH HHS R35 GM145252NINDS NIH HHS R43 NS127740
6 · The paper itselfAbstract
Ornithine decarboxylase (ODC) catalyzes the first committed step in polyamine biosynthesis and plays a central role in cellular growth and proliferation. Quantitative analysis of ODC activity has traditionally relied on radiometric or coupled-enzyme assays, which limit scalability and accessibility. Here, we report on the development of 1,2-diacetylbenzene (DAB)-ODC, a fluorescence-based assay that enables direct, sensitive, and high-throughput quantification of ODC activity by detecting putrescine through its reaction with DAB. Using purified recombinant ODC from Saccharomyces cerevisiae and humans, we show that DAB-ODC supports measurement of enzyme activity and accurate determination of steady-state kinetic parameters. Inhibition studies with the inhibitor DL-α-difluoromethylornithine and three recently reported DL-α-difluoromethylornithine analogs yielded IC
Indexed as
Enzyme AssaysEnzyme InhibitorsOrnithine DecarboxylaseOrnithine Decarboxylase InhibitorsDrug Evaluation, PreclinicalFluorescenceHumansKineticsPutrescineSaccharomyces cerevisiaeEnzyme InhibitorsOrnithine DecarboxylaseOrnithine Decarboxylase InhibitorsPutrescine1,2-diacetyl benzenecancerdrug discoveryenzyme activityfluorescence assayinhibitionisoindoleornithineornithine decarboxylasepolyaminesputrescinespermidinespermine
Identifiers
PMID42001943
PMCPMC13214536
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390