ArticleImmunoHorizons2026
Temporal variability in healthy donor IFN-γ ELISpot responses reveals a stable IL-7-responsive functional metric for immune phenotyping.
Article in ImmunoHorizons, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Functional T-cell assays such as the IFN-γ ELISpot are increasingly used for immune phenotyping in infection, cancer, and critical illness. Many studies, including recent work linking low IFN-γ ELISpot responses to adverse outcomes in sepsis, compare patient samples to a single cross-sectional healthy donor baseline, implicitly assuming temporal stability of IFN-γ secretion in health. We conducted a longitudinal study of 9 healthy adults using whole-blood anti-CD3/anti-CD28 ELISpot assays with and without IL-7 co-stimulation. Absolute IFN-γ production varied significantly within individuals over approximately 10 weeks, whereas the ratio of IL-7-augmented to anti-CD3/anti-CD28-only responses remained remarkably stable across all readouts. This IL-7 responsiveness ratio may represent a robust, time-independent functional metric of intact T-cell signaling. These findings demonstrate that absolute IFN-γ ELISpot values in healthy individuals are dynamic and support ratio-based metrics for immune phenotyping applications.
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