ArticleSTAR protocols2026
A protocol for single-sequence protein-RNA complex structure prediction using ProRNA3D-single.
Article in STAR protocols, 2026. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Not yet cited in PubMed.
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Abstract
Characterizing the interaction between protein and RNA is critically important for biology and medicine. Here, we present a protocol to predict three-dimensional protein-RNA complex structural models from their sequences using a deep learning-based approach named ProRNA3D-single. First, we delineate detailed steps for predicting protein-RNA interaction maps by leveraging pretrained language models. We then describe the procedure for generating the protein-RNA complex structure from the predicted interactions using optimization techniques. This protocol has potential applications in host-virus interactions and drug design. For complete details on the use and execution of this protocol, please refer to Roche et al.
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